固相萃取/LC-MS/MS测定血液中的白藜芦醇  

The determination of resveratrol in blood with LC-MS/MS by SPE

作　　者：李丽[1] 张文芳[2] 杨士云[2] 张大明[2] 常红发[3] 

机构地区：[1]山西医科大学法医学院,山西太原030001 [2]北京市公安局法医鉴定中心 法庭毒物分析公安部重点实验室,北京100192 [3]重庆市公安局刑警总队,重庆400024

出　　处：《中国法医学杂志》2012年第2期112-114,共3页Chinese Journal of Forensic Medicine

摘　　要：目的建立血液中白藜芦醇的固相萃取/LC-MS/MS方法。方法自制固相萃取柱,在长约13cm的酸性滴定管中,底部填入约0.02g玻璃棉,依次加入柱填料PSA 0.15g,Florisil 0.3g,Silica 0.45g。对样本进行固相萃取,采用LC-MS/MS方法进行检测,运用保留时间和多反应监测(MRM)方式对血液中白藜芦醇进行定性定量分析。结果回收率为95.66%～98.58%,最低检测限(LOD)为1.79μg/mL,线性范围2.5～20μg/mL;相关系数为0.9997。结论本文所建方法,适用于血液中白藜芦醇的检测。Objective To establish a solid phase extraction （SPE）/ LC-MS/MS method for determination of resveratrol in blood. Methods Made selfmade extraerion cartridges, added about 0.02g glass sponge in the bottom of a 13 centimeters long acid burette,then followed by the bottom to the top added about 0. 15g of PSA,0.3g of Florisil and 0.45g of Silica column packings. Resveratrol were extracted from human blood using selfmade extraction cartridges and detected by LC-MS/MS. Qualitative and quantitative analysis was obtained by retention time （Rt） and Multiple Reaction Monitoring （MRM） of LC-MS/MS. Results The recovery was 95.66% -98.58%. Limits of Detection （LOD） was 1. 79μg/mL. The linear range was 2.5 20μg/mL. The correlation coefficient was 0. 9997. Conclusion The method is applicable to the analysis of resveratrol in blood.

关 键 词：法医毒物分析 白藜芦醇 固相萃取 LC-MS/MS 血液 

分 类 号：D919[医药卫生—法医学]