Preparation and Characterization of Chitosan Microsphere Loading Bovine Serum Albumin  

作　　者：孙庆申 付宏刚 

机构地区：[1]Key Laboratory of Functional Inorganic Material Chemistry, Heilongjiang University, Ministry of Education [2]University Key Laboratory of Microbiology, College of Life Science, Heilongjiang University

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2012年第3期459-464,共6页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the National Natural Science Foundation of China (Nos.31000773 and 31072119);Key Scientific and Technological Planning Project of Harbin (No.2009AA6CN125);Innovation Foundation of Harbin (No.2010RFQXN091)

摘　　要：To optimize the preparation process of chitosan microspheres and study its loading capacity, chitosan microsphere was prepared by crosslinking with glutaraldehyde, and bovine serum albumin （BSA） was absorbed onto chitosan microsphere. Scanning electron microscope （SEM）, Fourier transform infrared spectroscopy （FITR）, TA instruments and zeta potentiometer analyzer were used to characterize the parameters with respect to size, thermal characters, morphology, and zeta potential of the microspheres. The loading capability and in vitro release tests were carried out. The results showed that chitosan microsphere with particle size less than 10 μm and positively charged （＋25.97±0.56 mV） can be obtained under the aldehyde group to amino group ratio at 1：1. A loading capacity of BSA at 28.63±0.15 g/100 g with corresponding loading efficiency at 72.01±1.44% was obtained for chitosan microsphere. In vitro test revealed a burst release followed by sustained-release profile.To optimize the preparation process of chitosan microspheres and study its loading capacity, chitosan microsphere was prepared by crosslinking with glutaraldehyde, and bovine serum albumin （BSA） was absorbed onto chitosan microsphere. Scanning electron microscope （SEM）, Fourier transform infrared spectroscopy （FITR）, TA instruments and zeta potentiometer analyzer were used to characterize the parameters with respect to size, thermal characters, morphology, and zeta potential of the microspheres. The loading capability and in vitro release tests were carried out. The results showed that chitosan microsphere with particle size less than 10 μm and positively charged （＋25.97±0.56 mV） can be obtained under the aldehyde group to amino group ratio at 1：1. A loading capacity of BSA at 28.63±0.15 g/100 g with corresponding loading efficiency at 72.01±1.44% was obtained for chitosan microsphere. In vitro test revealed a burst release followed by sustained-release profile.

关 键 词：chitosan microsphere bovine serum albumin CROSSLINKING 

分 类 号：TQ460.1[化学工程—制药化工]