黄连同工酶及相关逆境指标对UV-B的响应研究  

Response of Isozyme and Stress Indexes of Coptis chinensis to UV-B Radiation

作　　者：温泉[1] 张楠[1] 曹瑞霞[1] 周心渝[1] 唐娟[1] 吴能表[1] 

机构地区：[1]西南大学生命科学学院/三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《中药材》2012年第3期341-346,共6页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金项目(30500041);中央高校基本科研业务费专项资金资助项目(XDJK2009C195)

摘　　要：目的:探讨UV-B辐射胁迫下黄连的抗逆生理机制,为黄连栽培及提高黄连品质提供参考依据。方法:通过设置UV-B辐射时间及不同强度梯度,运用聚丙烯酰胺垂直板电泳技术,对各处理组的黄连同工酶及相关逆境指标进行了分析。结果:UV-B辐射3 h后,SOD1(Rf=0.125)、SOD2(Rf=0.312)、CAT1(Rf=0.428)、POD3(Rf=0.290)、POD4(Rf=0.636)谱带被先后诱导,随着UV-B辐射时间的增长,这些谱带开始丢失或减弱,尤其处于重度UV-B胁迫下,CAT1(Rf=0.428)、POD3(Rf=0.290)谱带会提前丢失;此外,UV-B辐射使得叶片中MDA、可溶性糖、脯氨酸含量显著高于对照组,但UV-B辐射7 h后,除UL组脯氨酸上升外,各组MDA、可溶性糖、脯氨酸含量开始有所下降,可溶性蛋白谱带数增多。结论:短时间的UV-B辐射会诱导黄连增加抗氧化酶同工酶的表达量及谱带数,积累可溶性糖、可溶性蛋白等抗性物质来提高自身抗性能力,UV-B辐射持续7 h后,黄连自身调节系统被破坏,代谢开始紊乱,同工酶谱带开始丢失及减弱,可溶性蛋白条带增多变宽。Objective：To study the physiological mechanism of anti-stress of Coptic chinensis and provide theoretical basis for its cultivation and promoting its quality.Methods：Different degrees of the range of time and intensity of UV-B radiation were set in the experiment.Used the technique of polyacrylamide gelatin vertical board electrophoresis（PAGE） to analyse the isozyme and related stress index.Results：The isoenzymic bands of SOD1（Rf=0.125）,SOD2（Rf=0.312）,CAT1（Rf=0.428）,POD3（Rf=0.290）,POD4（Rf=0.636）were induced by UV-B radiation after 3 hours,with the increase of the time of UV-B radiation,those isoenzymic bands was going to vanish or became unclear.Moreover,isoenzymic bands of CAT1（Rf=0.428）、POD3（Rf=0.290） disappeared in advance under heavy intensity of UV-B radiation.Furthermore,the contents of MDA,soluble sugar,proline were higher dramatically than those of control group under UV-B radiation.However,excluding the increases of proline in UL group,the content of MDA,soluble sugar,proline of other groups commenced to decrease slowly and isoenzymic bands of soluble protein increase after 7 hours of UV-B radiation.Conclusion：The increase of the expression of antioxidase isozyme,accumulation of soluble sugar,soluble protein and other antioxidase matter is induced by the short-time UV-B radiation,which can protect Coptis chinensis from being harmed by UV-B radiation.However,regulation system of Coptis chinensis are broken,metabolism is disordered,the bands of antioxidase isozyme vanish or weaken,the bands of soluble protein are increased and widened,these phenomenon is caused by 7 hours of UV-B radiation.

关 键 词：黄连 UV-B辐射 同工酶 聚丙烯酰胺凝胶电泳. 

分 类 号：R282.2[医药卫生—中药学]