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作 者:何报作[1] 覃继佳[1] 朱意麟[1] 廖月葵[1]
出 处:《中药材》2012年第3期385-391,共7页Journal of Chinese Medicinal Materials
基 金:国家自然科学基金资助项目<叶形态-脉序图谱鉴定中药的方法研究及其支持平台的建立>(30860371)
摘 要:目的:寻找马蓝叶与其易淆品路边青叶的鉴别特征,建立有效的鉴别方法。方法:LMVP(叶形态-脉序图谱鉴别法),QAERM(定量分析评价鉴别中药品种真伪方法的可靠性方法)。结果:主要区别点:马蓝叶的叶基渐狭下延,干叶深暗蓝绿色、暗棕绿色,具梭状小点,无腺点或局部有小腺点,叶柄与中脉的界限不明显,最末二级脉的下方有三级脉从中脉发出;路边青的叶基不下延,干叶黄绿色、绿色、绿黄色、黄棕色,无梭状小点,密布腺点,叶柄与中脉的界限明显,最末二级脉的下方无三级脉从中脉发出。同时以上述6组区别点为指标,鉴别结果的正确性(AC)=98.5%~99.2%;重复性为符合率(ARO)=97.7%,一致性强度(Kappa)=0.95。结论:建立的"叶形态-脉序图谱鉴别法"可准确鉴别马蓝叶及易淆品路边青叶。操作简便、快速、价廉、可靠性强。Objective: To study the identification characters of Baphicacanthus cusia and its confused herb Clerodendrum cyrtophyllum and establish an identification method.Methods: LMVP(leaf morphological-venation pattern for identification Chinese herbs)and QAERM(quantitatively analyze and evaluate reliability for the method of identification Chinese herbs) were applied for the study.Results: The main differences of the leaf were as follows:Baphicacanthus cusia: leaf base attenuate,dry leaves deep dull blue green,spindle spots,no or small glandular scales somewhere,the limit between midrib and petiole was not clear,below undermost secondary vein some tertiary veins issue from midrib;Clerodendrum cyrtophyllum: leaf base not attenuate;dry leaves yellow green,green,green yellow,yellow brown,no spindle spots,densely covered by glandular scales,the limit between midrib and petiole was clear,below undermost secondary vein none tertiary veins issue from midrib.With the mentioned six groups of key differences,the both plants could be successfully identified from each other.The accuracy of identification results(AC) was from 98.5% to 99.2%,the repeatability of identification results: agreement rate for observation(ARO) was 97.7% and Kappa value was 0.95.Conclusion: The established method is simple,rapid,economic and reliable.
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