阿魏酸钠缺血后处理对大鼠心肌缺血再灌注损伤的保护作用的研究  

The Study of the Cardioprotective Effect of Sodium Ferulate Postconditioning Against Myocardial Ischemia and Reperfusion Injury in Rat

作　　者：张帆[1] 游陆 袁德敏[3] 梁克纪[3] 张学玲[3] 杨新春[1] 

机构地区：[1]首都医科大学附属北京朝阳医院心脏中心,北京100020 [2]辽宁省丹东市第一人民医院心内科,丹东118000 [3]牡丹江市第一人民医院心内三科,牡丹江157011

出　　处：《中国医药导刊》2012年第2期288-289,280,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:探讨阿魏酸钠(SF)对大鼠心肌缺血再灌注损伤的保护作用及可能的机制。方法:40只Sprague-Dawley大鼠随机分为四组,A组:假手术组;B组:缺血/再灌注损伤组;C组:缺血后处理组;D组:SF后处理组。建立缺血后处理模型,实验结束后取出心脏,测定心肌梗死面积,TNF-α,IL-10的含量及核因子-κB的活性。结果:(1)心肌梗死面积的测定:与缺血/再灌注损伤组相比,SF后处理组明显减小了心肌梗死面积(P<0.05)。(2)心肌中TNF-α和IL-10含量的测定:TNF-α、IL-10在缺血再灌注组均升高,SF后处理组同缺血再灌注组比较TNF-α水平明显减低(P<0.05),IL-10含量则明显增高(P<0.05)。(3)NF-κB活性的测定:NF-κB活性在缺血再灌注组显著提高,与缺血再灌注组相比,SF后处理组显著阻止NF-κB活性的升高(P<0.05)。结论:适当剂量SF后处理可以减弱心肌缺血再灌注损伤,可能与其减轻NF-κB的活性,从而减弱肿瘤坏死因子-α的表达,减低由缺血再灌注损伤引起的炎症反应所致。Objective：To study the cardioprotective effect of Sodium Ferulate（SF） postconditioning against myocardial ischemia and reperfusion injury in rat.Methods：Forty Sprague-Dawley rats were divided into four groups.Hearts were removed at the termination of experiments,which were preserved in frozen tube and stored at-70oC refrigerator for measurement myocardial infarct size and activities of the NF-κB and TNF-α and IL-10 assay.Results：（1）Measurement of myocardial infarct size：myocardial infarct size in SF postconditioning group was obviously decreased than Group B（P0.05）.（2）Measurement of TNF-α and IL-10 assay：TNF-α and IL10 assay increased in Group B.TNF-α assay in SF postconditioning group was obviously decreased than Group B（P0.05）,but IL10 assay markedly increased（P0.05）.（3）Measurement activity of the NF-κB：activity of the NF-κB obviously increased in Group B.SF postconditioning markedly inhibited NF-κB activity compared to Group B（P0.05）.Conclusions：Based on the above results,we speculated that post treatment with an appropriate dose of SF could weaken myocardial I/R injury,which might in part be attributed to the desease of the NF-κB activity and TNF-α expression,accordingly reduced inflammatory caused by myocardial I/R injury.

关 键 词：阿魏酸钠 缺血后处理 核因子-ΚB 肿瘤坏子因子-α 白介素-10 

分 类 号：R285.5[医药卫生—中药学]