乙型肝炎病毒DNA定量分析的临床意义  

Hepatitis B Virus DNAQuantitativeAnalysis and Clinical Significance of

作　　者：刘兵[1] 胡蓉[1] 杨联云 陈亘志 黄志成[1] 

机构地区：[1]重庆市合川区人民医院检验科,重庆401520

出　　处：《中国医药导刊》2012年第4期673-674,共2页Chinese Journal of Medicinal Guide

摘　　要：目的:探讨乙型肝炎病毒HBV DNA含量与血清标记物的相关性及其对乙型肝炎诊断、预后的意义。方法:采用酶联免疫吸附实验(ELISA)法检测1652例乙肝患者血清学标记物(HBV—M),其血清学模型包括以下9种组合;并选取160例HBV M全阴性正常人作为对照。实时荧光定量PCR(FQ-PCR)检测血清中HBV DNA含量。结果:1、2组HBV DNA阳性率为98.4%和96.67%,明显高于其它组。结论:HBV DNA含量与乙肝病毒e抗原具有显著相关性,HBV M和HBV DNA定量检测对于临床乙肝病毒感染、复制及临床治疗有重要意义。Objective： To analyze the correlation of hepatitis B virus（HBV）DNA with hepatitis M,and to investigate their clinical signifcance in HBV diagnosis and prognosis.Methods： 1652 pauents were enrolled in this study and divided into four groups according to the quantitative analysis of serum biomarker HBV—M by enzyme linked immtmosorbent assay（ELISA）Patients in group.Fluoroimmune quantitative PCR（FQ—PCR）was used to measu the levels of HBV DNA and ELISA for detection.Results： Positive rate of HBV-DNA in group 1 and 2 was 98.4%.Significantly higher than that other groups.Conclusion： There is significant correlation between the lerels of HBV DNA and hepatitis B e antigen.Quantitative detection of both HBV-M and HBV DNA combined with may be available in the diagnosis of hepatitis B virus infection,republication and clinical treatment.

关 键 词：乙型肝炎病毒 乙肝肝炎病毒DNA HBVM FQ—PCR 

分 类 号：R512.62[医药卫生—内科学]