Microarray screening of differentially expressed genes in DCX transfected U87 cells before and after irradiation  

作　　者：FENG Shuang WANG Jie JIANG Xin XU Yuanyuan ZHANG Haowen Cao Jianping YU Jiahua LIU Fenju 

机构地区：[1]School of Radiation Medicine and Protection,Jiangsu Key Laboratory of Radiation Medicine and Protection,Medical College of Soochow University,Suzhou,215123,China

出　　处：《Nuclear Science and Techniques》2012年第2期97-102,共6页核技术（英文）

基　　金：Supported by National Natural Science Foundation of China (NO.30870585);the Priority Academic Program development of Jiangsu Higher Education Institutions (PAPD)

摘　　要：Radiation therapy plays a critical role in the treatment of neurogliocytoma and it is known that doublecortin (DCX)-transfected U87 cells can inhibit tumor cell growth. Microarray analysis to screen for differentially expressed genes in DCX-transfected U87 cells before and after radiation uncovered DCX-related genes, the functions of DCX, and downstream genes in radiation therapy of neurogliocytoma. Stably transfected U87 cells were constructed (DCX-U87) and the differentially expressed genes were screened by microarray analysis to compare U87 cells with DCX-U87 cells in both non-irradiated and irradiated conditions. Cells were irradiated using 60Co γ-ray at a dose rate of 1.0 Gy/min. Mean values were subject to paired comparison analysis and genes with a p-value of less than 0.05 were analyzed. Differentially expressed genes can correlate with radiation sensitivity and DCX transfection. DCX and SPN proteins in DCX-U87 cells were detected by two groups of 0 and 10 Gy, but not the U87 cells, and their expression levels were higher in the 10 Gy group than in the 0 Gy group. The differential gene expression in DCX-U87 cells before and after radiation is helpful for future investigations into the mechanisms of radiation therapy in neurogliocytoma cells.Radiation therapy plays a critical role in the treatment of neurogliocytoma and it is known that doublecortin （DCX）-transfected U87 cells can inhibit tumor cell growth. Microarray analysis to screen for differentially expressed genes in DCX-transfected U87 cells before and after radiation uncovered DCX-related genes, the functions of DCX, and downstream genes in radiation therapy of neurogliocytoma. Stably transfected U87 cells were constructed （DCX-U87） and the differentially expressed genes were screened by microarray analysis to compare U87 cells with DCX-U87 cells in both non-irradiated and irradiated conditions. Cells were irradiated using 6~Co 7-ray at a dose rate of 1.0 Gy/min. Mean values were subject to paired comparison analysis and genes with a p-value of less than 0.05 were analyzed. Differentially expressed genes can correlate with radiation sensitivity and DCX transfection. DCX and SPN proteins in DCX-U87 cells were detected by two groups of 0 and 10 Gy, but not the U87 cells, and their expression levels were higher in the 10 Gy group than in the 0 Gy group. The differential gene expression in DCX-U87 cells before and after radiation is helpful for future investigations into the mechanisms of radiation therapy in neurogliocytoma cells.

关 键 词：基因芯片技术 基因差异表达 肿瘤细胞 射线照射 X架构 转染 DC 筛选 

分 类 号：Q78[生物学—分子生物学] S512.1[农业科学—作物学]