内毒素血症中肝细胞凋亡与Caspase-3蛋白表达的关系及阳离子A拮抗保护作用的研究  

The expression of Caspase-3 in liver cell apoptosis caused by endotoxin and the antagonized by Cation A in rats

作　　者：高洪[1] 屈常林[1] 高利波[1] 陈利平[1] 

机构地区：[1]云南农业大学动物科技学院,云南昆明650201

出　　处：《中国预防兽医学报》2012年第6期444-448,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：云南省高校科技创新团队支持计划(云教科[2011]14号);国家自然科学基金(30560113);云南省现代农业生猪产业技术体系建设(云财农2009[171]号);云南省教育厅科学研究项目(2010C255)

摘　　要：为研究内毒素血症中肝细胞凋亡与Caspase-3蛋白表达的关系及阳离子A(CA)的拮抗保护作用,本研究将72只SD大鼠随机分为对照组、内毒素(ET)组和CA保护组。3组动物经相应处理后分别在第3 h、4 h、8 h和12 h采集肝脏样品,采用流式细胞仪检测肝细胞凋亡情况、免疫组织化学染色技术检测Caspase-3蛋白的表达情况。结果显示,凋亡肝细胞百分比ET组极显著高于对照组和CA保护组,ET组肝细胞凋亡百分比呈上升趋势,CA保护组在3 h、4 h和8 h 3个时间点呈上升趋势,在12 h呈下降趋势。ET组Caspase-3表达极显著高于对照组和CA保护组。研究表明,在内毒素血症中肝细胞凋亡与Caspase-3蛋白的表达呈正相关,而CA则能够明显下调Caspase-3在肝脏的表达水平,从而抑制肝细胞凋亡,对由ET诱导的肝损伤发挥保护作用。To explore the relationship between apoptosis of liver cells and Caspase-3 in endotoxemia （ET） and antagonistic effect of Cation A （CA） in rats. Seventy-two adult healthy SD rats were randomly divided into 3 groups, including control group, ET group and CA group. Hepatic tissues in three groups were collected for tests at 3rd, 4th, 8th and 12th hour after treatment. Apoptosis of liver cells were detected by flow cytometry, and Caspase-3 expression was detected by immunohistochemical staining. The results showed that the percentage of apoptosis of liver cells in ET group were significantly higher than that of control and CA group. The percentage of hepatocyte apoptosis of ET group was in an upward trend, however, which was upward trend in the CA group at the 3rd, 4th, 8th hours time points, and went downward trend at the 12th hour. Caspase-3 expression in ET group was significantly higher than that of CA group. These results suggested that apoptosis and Caspase-3 protein expression in liver cell was positive correlation in endotoxemia, and CA have a significantly antagonistic effect on inflammatory injure through down-regulation of the expression of Caspase-3 pathway in liver, inhibiting liver cell apoptosis

关 键 词：内毒素 细胞凋亡 CASPASE-3 阳离子A 保护效应 

分 类 号：S852.33[农业科学—基础兽医学]