应用定量RT-PCR对猪瘟兔化弱毒疫苗的质量评价研究  

Qualification of the C-strain-based vaccines against classical swine fever using quantitative RT-PCR

作　　者：韩文[1] 王楠[1] 张兴娟[1] 葛叶[1] 韩秋颖[1] 孙元[1] 李素[1] 仇华吉[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2012年第6期464-467,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技支撑计划(2006BAD06A03)

摘　　要：为进一步验证猪瘟兔化弱毒(HCLV)疫苗荧光定量RT-PCR(qRT-PCR)与兔体定型热试验之间存在正相关性,本研究利用qRT-PCR方法对278批次HCLV疫苗进行检测,分别检测其疫苗含量及牛病毒性腹泻病毒(BVDV)污染情况。结果表明,278批次猪瘟疫苗中有66批(24%)疫苗含量达不到规程标准,有42批(15%)疫苗存在BVDV污染;从所检疫苗中抽取6份qRT-PCR检测HCLV含量较低的疫苗,采用兔体定型热试验进行验证,两种方法结果吻合,表明qRT-PCR方法可以作为评价猪瘟疫苗质量的一种备选方法。Our previous studies showed that positive correlation existed between the quantitative RT-PCR （qRT-PCR） detection and body fever response method in rabbits for quality assay of classical swine fever virus （CSFV） C-strain-based vaccine （HCLV）. In this study, we detected 278 batches of finished products of HCLV from 20 manufactures using the qRT-PCR for detecting the virus contents and bovine viral diarrhoea virus （BVDV） contaminant. The results indicated that 24% （66/278） of vaccine batches was under the national standard for the virus contents, and 15% （42/278） of which was contaminated with BVDV. Furthermore, the results of body temperature responses in rabbits were consistent with the qRT-PCR results on 6 selected vaccine batches, which demonstrated qRT-PCR could be an alternatives method to be used for quality control assay in process of HCLV production.

关 键 词：猪瘟兔化弱毒疫苗 实时荧光定量RT-PCR 牛病毒性腹泻病毒 疫苗质量 

分 类 号：S852.65[农业科学—基础兽医学]