首参颗粒对动脉粥样硬化大鼠血管细胞与外周血白细胞端粒和端粒酶的影响  

Effects of Chinese herbal medicine Shoushen Granule on telomere length and telomerase activity of peripheral white blood cells and vascular cells in rats with atherosclerosis

作　　者：陈川[1] 迟惠英[1] 郁志华[1] 陈久林[1] 

机构地区：[1]上海市中医老年医学研究所,上海200032

出　　处：《中西医结合学报》2012年第6期667-673,共7页Journal of Chinese Integrative Medicine

基　　金：上海市教育委员会预算内科研项目 (No09JW45);上海市卫生局局级科研项目(No2010225);上海市卫生局中医药科研基金项目(No2010L046A)

摘　　要：目的:观察补肾中药复方首参颗粒对动脉粥样硬化(atherosclerosis,AS)模型大鼠血管细胞与外周血白细胞端粒、端粒酶以及动脉壁病变、血脂的影响,探讨其作用机制。方法:40只Sprague-Dawley大鼠,随机分为正常组、模型组、中药组和西药组;采用一次性腹腔注射维生素D3及连续饲喂高脂饮食的方法建立AS模型。模型组大鼠用双蒸水灌胃,中药组以首参颗粒灌胃,西药组以普伐他汀灌胃。12周后,苏木精和伊红染色观察腹主动脉髂动脉病理变化,生物化学比色法检测血清总胆固醇(total cholesterol,TC)、三酰甘油(triacylg-lycerol,TAG)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)和低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)水平,荧光定量聚合酶链反应法检测外周血白细胞及血管细胞的端粒长度及端粒酶活性。结果:与模型组比较,中药组大鼠动脉壁的粥样硬化病变明显改善,血清TC、LDL-C水平及TC/HDL-C比值显著降低(P<0.01),血管细胞与外周血白细胞的端粒酶活性明显提高(P<0.05或P<0.01),血管细胞与外周血白细胞的端粒长度延长,与正常组比较差异无统计学意义(P>0.05)。结论:补肾中药复方首参颗粒具有改善大鼠AS的作用,其机制可能与调控血管细胞端粒长度及端粒酶活性,改善血管细胞老化相关。OBJECTIVE： To observe the effects of Shoushen Granule, a compound traditional Chinese herbal medicine, on telomere length and telomerase activity in peripheral leukocytes and vascular cells, artery wall lesions and blood lipid in a Sprague-Dawley （SD） rat model of atherosclerosis. METHODS： Forty SD rats were randomly divided into normal control group, model group, Shoushen Granule group and Western medicine group with 10 in each group. The rat model of atherosclerosis was established by high-fat diet and vitamin D3 loading. The model group was given gastric perfusion of double distilled water; The Shoushen Granule group and the Western medicine group were respectively given gastric perfusion of Shoushen Granule and pravastatin. After 12 weeks, pathological changes of abdominal aorta were determined by hematoxylin and eosin staining. Biochemical colorimetric method was used to detect the contents of total cholesterol （TC）, triacylglycerol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol （LDL-C） in serum of the rats. Telomere length and telomerase activity in peripheral leukocytes and vascular cells of the rats were tested by quantitative real-time polymerase chain reaction method. RESULTS： When compared with the model group, atherosclerosis lesions of the arterial wall were significantly improved in the Shoushen Granule group. In addition, both TC and LDL-C levels in the Shoushen Granule group were decreased significantly compared with the model group （P〈0.01）. Besides, not only telomerase activity but also telomere length in peripheral leukocytes （P〈0.01） and vascular cells （P〈0.05） were increased significantly as compared to those in the model group. However, there was no significant difference between the Shoushen Granule group and the normal control group （P〉0.05）. CONCLUSION： Shoushen Granule improves the atherosclerosis lesions in rats, and the mechanism may be related to regulating telomere length and telomerase activity.

关 键 词：中草药 动脉粥样硬化 端粒 端粒 末端转移酶 血脂异常 大鼠 

分 类 号：R285.5[医药卫生—中药学]