肝囊型包虫病患者外周血单个核细胞表面程序性死亡受体配体1的表达及其与干扰素-γ的关系  

Expression of programmed death receptor ligand 1 of peripheral blood mononuclear cells in patients with hepatic cystic echinococcosis and its relation with interferon-γ

作　　者：巫姜[1,2] 李涛[1,2] 张志[1,2] 赵晋明[2] 王俊华[1] 张雪[3] 林仁勇[1] 温浩[1,2] 

机构地区：[1]新疆医科大学第一附属医院新疆包虫病基础医学重点实验室,乌鲁木齐830054 [2]新疆医科大学第一附属医院消化血管外科中学,乌鲁木齐830054 [3]新疆医科大学第一附属医院医学研究中心,乌鲁木齐830054

出　　处：《中华消化外科杂志》2012年第3期275-278,共4页Chinese Journal of Digestive Surgery

基　　金：国家自然科学基金（30960342）;新疆维吾尔自治区高技术研究与发展计划项目（200810104）;新疆包虫病基础医学重点实验室开放课题资助项目（XJDX0202-2008-05）

摘　　要：目的探讨肝囊型包虫病患者外周血单个核细胞（PBMC）表面程序性死亡受体配体1（PD—L1）的表达及其与血清IFN-γ水平之间的关系。方法回顾性分析2010年6月至2011年2月新疆医科大学第一附属医院收治的63例肝囊型包虫病患者的临床资料。根据世界卫生组织包虫病专家组包虫病标准化分型原则将患者分为肝包虫活性组（38例）和肝包虫无活性组（25例），20例肝血管瘤患者及健康志愿者作为正常对照组。采用流式细胞仪及免疫组织化学法检测PD—L1阳性表达率，ELISA法检测血清IFN-γ的表达水平。组间比较采用成组设计资料t检验和单因素方差分析，进一步两两比较采用LSD检验，总体分布采用X2检验，PD—L1阳性表达率和IFN-γ的表达水平的相关性分析采用Pearson检验。结果流式细胞仪检测肝包虫活性组、肝包虫无活性组和正常对照组PBMC表面PD—L1的阳性表达率分别为12．1％±3．8％、10．9％±2．5％和9．1％±2．5％，肝包虫活性组与正常对照组PD—L1的阳性表达率比较，差异有统计学意义（t=3．327，P〈0．05）。免疫组织化学检测肝包虫活性组、肝包虫无活性组和正常对照组PBMC涂片中PD—L1的阳性表达率分别为11．9％±3．4％、10．6％±2．9％和9．5％±3．6％，肝包虫活性组与正常对照组PD—L1的阳性表达率比较，差异有统计学意义（t=2．470，P〈0．05）。肝包虫活性组、肝包虫无活性组和正常对照组血清IFN—γ表达分别为（141±38）μg/L、（124±32）μg/L和（105±42）μg/L，肝包虫活性组与正常对照组IFN-γ表达水平比较，差异有统计学意义（t=3．280，P〈0．05）。流式细胞仪和免疫组织化学检测PBMC的PD-L1阳性表达率与血清IFN-γ表达水平均呈明显正相关（r=0．59，0．61，P〈0．05）。结论PD—L1可能在IFN-γ的作用下发挥着促进肝包虫免疫逃避的重要作用。Objective To investigate the expression of programmed death receptor ligand 1 （ PD-L1 ） of peripheral blood mononuelear cells （PBMCs） in patients with hepatic cystic echinococcosis （HCE） and its relation with interferon-γ Methods The clinical data of 63 patients with HCE who were admitted to the First Affiliated Hospital of Xinjiang Medical University from June 2010 to February 2011 were retrospectively analyzed. All patients were divided into HCE active group （38 patients） and HCE non-active group （25 patients） according to the system established by the World Health Organization＇s Informal Working Group on Echinococcosis. Twenty patients with hepatic hemangioma or healthy individuals were recnlited in normal control group. The positive rate of PD-L1 expression was detected by flow cytometry and immunocytoehcmistry. The expression of interferon-γ was detected by enzyme-linked immunosorhent assay （ELISA）. All data were analyzed by the t test, one-way analysis of variance, LSD test and chi-square test. The relationship between the expression of interferon-γ and positive rate of PD-L1 expression was analyzed by the Pearson test. Results The results of flow eytometry showed that the positive rates of PD-L1 expression in the HCE active group, HCE non-active group and normal control group were 12.1%±3.8%, 10.9%±2.5% and 9.1%±2.5%, respectively. There was a significant difference in the positive rate of PD-L1 expression between the HCE active group and normal control group （t = 3. 327, P 〈 0.05 ）. The results of imnmnohistochemistry showed that the positive rates of PD-L1 expression in the HCE active group, HCE non-active group and normal control group were 11.9%±3.4%, 10.6%±2.9% and 9.5%±3.6%, respectively. There was a significant difference in the positive rate of PD-L1 expression between the HCE active group and normal control group （ t = 2. 470, P 〈 0.05）. The expressions of interferon- in the HCE active group, HCE non-active group and normal control group were （141 �

关 键 词：细粒棘球蚴病 程序性死亡受体配体1 流式细胞术 免疫组织化学法 

分 类 号：R657.3[医药卫生—外科学]