上呼吸道分离鲍曼不动杆菌1型整合子的分离与鉴定(英文)  

Characterized of class Ⅰ integron in Acinetobacter baumannii isolated from upper respiratory tract

作　　者：毛璞[1] 傅威[2] 叶丹[1] 杨淳[2] 单靖岚[1] 林美仪[1] 李常安[1] 刘晓青[2] 黎毅敏[2] 

机构地区：[1]广州医学院第一附属医院医院感染管理科,广州510120 [2]广州呼吸疾病研究所,呼吸疾病国家重点实验室,广州510120

出　　处：《微生物学报》2012年第6期791-796,共6页Acta Microbiologica Sinica

基　　金：Supported by the Program for Innovative Research Team of Education Bureau of Guangzhou(B94117);the Science and Technology project of Guangzhou(2010J-E171)~~

摘　　要：【目的】研究I型整合子的结构特征,探讨其与细菌多重耐药之间的相关性。【方法】收集2008年至2009年广州呼吸疾病研究所上呼吸道分离的187株鲍曼不动杆菌,应用K-B纸片扩散法检测耐药性,采用聚合酶链式反应进行I型整合子整合酶基因的检测;扩增整合子的可变区,应用DNA测序技术分析I型整合子基因结构。【结果】I型整合子的阳性率达53.4%。共七种1型整合子基因盒被鉴定,其中首次发现报道一种新的整合子(GenBank:HQ322622)。可变区主要编码氨基糖苷类药物的耐药基因。20种抗菌素耐药的结果均表明携带Ⅰ型整合子的鲍曼不动杆菌耐药率较不携带I型整合子的鲍曼不动杆菌的耐药率明显增高。整合子与鲍曼不动杆菌的多重耐药表型具有密切相关性。【结论】I类整合子相关耐药基因在本院临床分离鲍曼不动杆菌中分布较广泛。整合子在鲍曼不动杆菌耐药性的形成和播散中具有重要作用。[ Objective] To characterize the class I integron in Acinetobacter baumannii and to analyze the correlation between integron and drug resistance. [ Methods] In total 187 strains were collected between 2008 and 2009. All strains were tested by Kirb-Bauer disk diffusion test for drug resistance. PCR and DNA sequencing were used to detected class I integrase gene and to clarify the context of gene cassette. [ Results ] Class I integrase gene was detected in 100 （53.4%） of the isolates analyzed. Seven different gene cassettes were identified, including a new integron （GenBank： HQ322622） carrying an unknown protein probably associated with recombination. The vast majority of the cassettes encoded amonoglycoside resistance gene, including aacA4, aadA1, aacC1, aac6 ]I, aadA2. Susceptibility data show that strains carrying class I integron were significantly more resistant to all of the antibiotics tested than isolates lacking class I integron. The correlation between the presence of integron and the multidrug-resistance of A. baumannii was statistically significant. [ Conclusion] Drug resistance genes integrated by Class ] integron were widespread in A. baumannii. Class I integron plays an important role in resistance of A. baumannii.

关 键 词：多重耐药 鲍曼不动杆菌 Ⅰ型整合子 

分 类 号：R378[医药卫生—病原生物学]