地塞米松对哮喘豚鼠血清白细胞介素-4变化及肺组织形态的影响  

Effects of Dexamethasone on Serum IL-4 and the Pathological Changes of Lung Tissue of Guinea Pig with Asthma

作　　者：罗光伟[1] 徐玮[2] 吴霜[3] 万军[1] 黄燕玲[1] 杨硕[1] 张念[1] 徐清[1] 孙洁民[1] 肖宏[4] 

机构地区：[1]武汉市中西医结合医院呼吸内科,湖北武汉430022 [2]湖北中医药大学,湖北武汉430065 [3]云南中医学院,云南昆明650500 [4]武汉市中西医结合医院中心实验室,湖北武汉430022

出　　处：《现代生物医学进展》2012年第10期1828-1830,1819,共4页Progress in Modern Biomedicine

基　　金：武汉市卫生局资助课题(武卫[2001]235号)

摘　　要：目的:观察地塞米松对哮喘豚鼠血清白细胞介素-4(IL-4)及肺组织形态的影响,探讨糖皮质激素治疗哮喘的机制。方法:30只健康雄性Hartley系豚鼠随机分为空白对照组,病理模型组,地塞米松组。用卵白蛋白(OVA)复制哮喘豚鼠模型。采用流式细胞术分析CD4+interleukin-4(IL-4)细胞占CD4+T细胞的比例,光镜检查肺组织形态变化。结果:病理模型组外周血中IL-4明显高于空白对照组(P<0.05),地塞米松组外周血中IL-4较病理模型组明显降低(P<0.05),且地塞米松组支气管上皮损伤,粘液腺增生等病理改变较病理模型组明显改善。结论:地塞米松治疗哮喘的作用与抑制Th2的活化,改善支气管上皮损伤,粘液腺增生等病理改变有关。Objective： To observe the influence of dexamethasone on serum IL-4 and the pathological changes of lung tissue of guinea pig with asthma.and to explore the mechanism of dexamethasone in the treatment of asthma.Methods： 30 healthy male Hartley guinea pigs were divided into three groups randomly and equally： control group,model group,dexamethasone treatment group.The model guinea pigs of asthma was induced by intraperitoneal injection of ovalbumin.The level of CD4＋ IL-4 was tested by flow cytometer and analyzed statistically.Lung histological changes were examined by HE stains and under microscopy.Results： IL-4 increased obviously in model group compared with control group（P〈0.05）.The levels of IL-4 in serum of dexamethasone treatment groups were significantly lower than those of model group（P〈0.05）.The bronchial tube epider-mis damage and the mucous membrane gland proliferation were obviously improved in dexamethasone treatment group compared with model group.Conclusion： Dexamethasone could relieving asthma according to inhibiting the activation of Th2 and improveing the lung tectology changes.

关 键 词：地塞米松 哮喘 豚鼠 IIL-4肺组织病理 

分 类 号：Q95-3[生物学—动物学] R562.25[医药卫生—呼吸系统]