东亚钳蝎粗毒蛋白质热稳定性的研究  

Thermal Stablility Of Scorpion Venom Proteins From Bathus Martiensii(BMSV)

作　　者：倪伊婷[1] 马伟康[1] 齐先福[1] 张连祺[1] 王君德[2] 辛毅[1] 

机构地区：[1]大连医科大学生物技术系,辽宁大连116044 [2]大连医科大学国有资产管理处,辽宁大连116044

出　　处：《现代生物医学进展》2012年第10期1846-1848,共3页Progress in Modern Biomedicine

摘　　要：目的:通过蝎毒热溶解性和毒性实验,探索蝎毒蛋白稳定性与分子量间的关系。方法:对蝎毒粗毒煮沸后的可溶成分进行急性毒力实验和SDS-PAGE电泳分析。结果:蝎毒粗毒加热处理组的LD50为16.228mg/kg,粗毒对照组的LD50为3.66mg/kg,显示蝎毒经加热后毒力明显降低,但仍具有一定的毒性。SDS-PAGE结果显示,经加热处理的粗蝎毒之沉淀主要为大分子蛋白质,在可溶成分中含有小分子肽类。结论:蝎毒的大分子蛋白质热稳定性差,而小分子多肽部分耐热性强,而且蝎毒的小分子多肽组分具有相对稳定的毒性。Objective： To investigate the relationship between the thermal stablility and protein molecular weight of scorpion venom from Bathus martiensii（BMSV） by detecting the solubility and toxicity after boilling.Methods： Boiled and non-boiled BMSV were centrifuged,and the supernatants were detected by SDS-PAGE analysis and toxicity detection.Results： Supernatant from boiled BMSV showed higher LD50 than that from non-bioled BMSV,and most proteins with high molecular weight were preciapteted and exhibated low toxicity.Supernatant from boiled samples maintained certain toxicity.Conclusion： The proteins with hjgh molecular weight from BMSV had low thermal stablility while the small peptides from BMSV had high thermal stablility.

关 键 词：蝎毒 热稳定性 聚丙烯酰胺凝胶电泳 半数致死量(LD50) 

分 类 号：Q95-3[生物学—动物学] R285[医药卫生—中药学]