耐甲氧西林金黄色葡萄球菌对万古霉素的MIC值的5年监测  

Methicillin-Resistant Staphylococcus Aureus to Vancomycin Values for 5 Years

作　　者：王珏[1] 陈友华[1] 成祥君[1] 文怡[1] 梅亚宁[1] 

机构地区：[1]南京医科大学第一附属医院,江苏南京210029

出　　处：《现代生物医学进展》2012年第10期1902-1905,共4页Progress in Modern Biomedicine

基　　金：江苏省实验诊断学重点实验室重大课题(C编号XK2007317)

摘　　要：目的:了解我院5年来耐甲氧西林金黄色葡萄球菌(MRSA)对万古霉素敏感性的变化,为临床治疗和控制该类感染提供依据方法:采用以肉汤稀释法测定近5年耐甲氧西林金黄色葡萄球菌(MRSA)临床分离株对万古霉素的最低抑菌浓度(MIC),按NC-CLS/CLSI2010年标准判定结果,WHONET5.5软件进行数据分析。结果:MRSA检出率从2005年的19.23%上升到2009年的42.14%,万古霉素的M IC几何均数从0.54μg/ml上升到1.21μg/ml,未检出万古霉素、替考拉宁耐药株,但2007年有2株,2008年、2009年各有1株菌株对替考拉宁呈中介,抑菌圈直径依次为12 mm、12 mm、13 mm、12 mm,MIC值均为16.0μg/ml。结论:MRSA检出率逐年上升,MRSA对万古霉素MIC值有升高的趋势,加强金黄色葡萄球菌对万古霉素敏感性的监测非常必要。Objective： To review and analyze the evolution of the MICs of vancomycin in methicillin-resistant Staphylococcus aureus（MRSA） strains isolated in our hospital during an five-year period to guide rational use of antimicrobial agents.Methods： The MICs of vancomycin were detected by broth macrodilution method on methicillin-resistant Staphylococcus aureus（MRSA） strains isolated from clinical specimens dring last 5 years,etermination of the results observed the standard of CLSI version 2010,and drug sensitivity analysis adopted WHONET5.5.Results： MRSA detection rate from 19.23 % in 2005 rose to 42.14 % in 2009,MICs geometri mean of vancomycin increased from 0.54 μg/ml to 1.21 μg/ml,No isolate was found drug resistanceto vancomycin and teicoplanin,Four strain of MRSA from 2007 to 2009 was intermediate to teicoplanin,The diameters（mm） of the inhibition zone were 12.0,12.0,13.0,12.0;The M ICs of vancomycin to 4 isolates were 4μg/ml.Conclusion： MRSA microorganisms are increasing from 2007 to 2009,MICs of vancomycin in MRSA indicated a trend of increasing;It is important to monitor the antimicrobial susceptibility of Staphylococcus aureus to vancomycin.

关 键 词：耐甲氧西林金黄色葡萄球菌 万古霉素 最低抑菌浓度 

分 类 号：R379[医药卫生—病原生物学]