变应性鼻炎鼻黏膜组织重塑的研究及转化生长因子β1在鼻黏膜中的表达  

The Study of the Remodeling and the Protein Expression of Transforming Growth Factorβ1 in the Nasal Mucosa of Allergic Rhinitis

作　　者：冯会杰[1] 周焕[1] 杨扬[1] 袁亚[2] 白素娟[1] 

机构地区：[1]上海市普陀区人民医院耳鼻咽喉科,上海200060 [2]上海市普陀区人民医院病理科,上海200060

出　　处：《现代生物医学进展》2012年第11期2151-2154,共4页Progress in Modern Biomedicine

基　　金：上海市普陀区卫生局科研课题基金资助(PWG-06-04)

摘　　要：目的:探讨变应性鼻炎(allergic rhinitis AR)鼻黏膜组织是否存在重塑并检测与组织重塑密切相关的转化生长因子β1(TGF-β1)在AR患者鼻黏膜组织中的表达及意义。方法:取健康自愿者、轻度间歇性AR患者、重度持续性AR患者的中鼻甲黏膜组织各10例。苏木素伊红(HE)染色法观察嗜酸细胞浸润并测定上皮损伤情况;阿辛蓝-过碘酸-希夫(AB-PAS)染色法计数杯状细胞数;三色胶原(MT)染色测定细胞外基质沉积面积百分比。酶联免疫吸附试验(ELISA)测定组织中TGF-β1的表达。结果:①对照组无明显嗜酸细胞浸润,两鼻炎组较多嗜酸细胞浸润(P<0.01),②轻度AR组中仅上皮细胞损伤1级比对照组明显(P<0.01),重度AR组上皮损伤1、2、3级均比对照组明显(P<0.01),③两鼻炎组杯状细胞数明显多于对照组(P值均<0.01),④与对照组相比,轻度AR组胶原沉积面积增多,但无统计学意义(P>0.05),重度AR组明显增多(P<0.01),⑤TGF-β1在两鼻炎组黏膜中的表达均比对照组显著增高(P<0.01);重度AR组TGF-β1的表达均比轻度AR组增高,具有统计学意义(P<0.05)。结论:AR的鼻黏膜组织发生了重塑,表现为:上皮细胞损伤,杯状细胞化生,细胞外基质沉积,重度AR患者的鼻黏膜重塑更强,更广泛。TGF-β1积极参与了AR鼻黏膜组织的重塑过程。Objective： To explore whether there was remodeling and the protein expression of transforming growth factor β1（TGF-β1） in the nasal mucosa of allergic rhinitis（AR） patients.Methods： Pathology staining explore the middle turbinate tissues from 10 patients with light-AR,from 10 patients with heavy-AR,from 10 non-AR.The infiltrating of eosinophils and damage of epithelium were examined by the hematoxylin and eosin staining（HE）;goblet cell hyperplasia were counted by the alcian blue-periodic acid-Schiff staining（AB-PAS）;the percentage area of extracellular matrix（ECM） deposition were used by the masson＇s Trichrome Staining（MT）;the protein expression of TGF-β1 in the middle turbinate tissues was detected by the enzyme-linked immunosorbent Assay（ELISA）.Results： Compared with the control group significant eosinophil infiltration and goblet cells was observed in both AR groups（P〈0.01）.Evident epithelial damage and ECM deposition were observed in heavy-AR group（P 0.01）.The expression of TGF-β1 in AR tissues was signif-icant higher（P〈0.01）.Conclusions： The nasal mucosa remodeling were observed in AR groups： eosinophils infiltration,epithelial dam-age,goblet cells hyperplasia and ECM deposition.TGF-β1 may play a role in the tissue remodeling processes.

关 键 词：变应性鼻炎 嗜酸细胞 上皮细胞 杯状细胞 细胞外基质 转化生长因子Β1 重塑 

分 类 号：R76[医药卫生—耳鼻咽喉科]