脓毒症导致小鼠胸腺萎缩的原因探讨  

Research on mechanisms of thymus atrophy induced by sepsis

作　　者：张卫梅[1] 孔雅娴[1] 栗雅杰[1] 孟庆才[1] 曾辉[1] 

机构地区：[1]首都医科大学附属北京地坛医院传染病研究所,北京100015

出　　处：《中华实验和临床感染病杂志（电子版）》2012年第2期8-10,共3页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：国家自然科学基金(81101251)

摘　　要：目的利用小鼠盲肠结扎穿刺模型探讨脓毒症导致胸腺萎缩的原因。方法采用盲肠结扎穿刺(CLP)手术建立脓毒症小鼠模型(脓毒症组),以假手术(Sham)小鼠作为对照组。术后7d,采用流式细胞仪检测小鼠胸腺中各群胸腺细胞和胸腺前体细胞比例的变化及胸腺细胞凋亡情况。结果与对照组相比,脓毒症组小鼠的胸腺明显萎缩,胸腺细胞的绝对计数显著减少(P<0.001);胸腺中的CD4-CD8-细胞(DN)、CD4+CD8+细胞(DP)、CD4+CD8-细胞、CD8+CD4-细胞数亦明显减少(P<0.001);胸腺中Lin-CD44+CD117+前体细胞比例显著降低(P<0.001);各群胸腺细胞凋亡的比例均显著增加(P<0.001)。结论脓毒症模型小鼠胸腺萎缩不仅与胸腺细胞凋亡增加有关,与胸腺前体细胞减少也相关。Objective To investigate the mechanisms of thymus atrophy induced by sepsis with mouse model which received caecal ligation and puncture （CLP）. Methods The model of sipsis was made by CLP and sham operation was taken as the control group.The changes of different stages of T lymphocyte and T lymphocyte progenitors in thymus were analyzed with flow cytometer 7 days after CLP. The apoptosis of T lymphocytes was analyzed with Annexin-Ⅴ staining. Results Compared with the control group, the thymus of sepsis mice atrophied obviously, accompanied by a significant decrease of thymocyte counts in different stages including CD4-CD8- （double-negative, DN）, CD4＋CD8＋ （double-positive, DP）, CD4＋CD8- and CD8＋CD4- thymocytes （P 0.001）. Meanwhile, sepsis mice showed an increased percentage of Annexin＋ thymocytes （P 0.001）. Moreover, decreased frequency of T lymphocyte progenitor cells （Lin-CD44＋CD117＋） was observed in the thymus of sepsis mice （P0.001）. Conclusions Sepsis-induced thymus atrophy was not only related to the increased proportion of apoptosis of thymocytes, but also to the decrease of T lymphocyte progenitors.

关 键 词：脓毒症 胸腺萎缩 淋巴细胞 细胞凋亡 胸腺前体细胞 

分 类 号：R459.7[医药卫生—急诊医学]