HBV表面抗原大蛋白与人胰腺突触角蛋白6之间的相互作用  

Confirmation of the interaction between hepatitis B virus large surface protein and homo sapiens pancreatic syntaxin 6 protein

作　　者：张海栋[1,2] 张锦前[1] 赵龙凤[2] 贾因棠[2] 王琦[1] 刘顺爱[1] 温少芳[1] 孙荣华[1] 李卓[1] 成军[1] 

机构地区：[1]首都医科大学附属北京地坛医院传染病研究所,北京100015 [2]山西医科大学第一医院感染病科

出　　处：《中华实验和临床感染病杂志（电子版）》2012年第2期22-24,共3页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：国家自然科学基金(30600524);太原市科技局大学生创新创业基金(201020-1720)

摘　　要：目的验证HBV表面抗原大蛋白(LHBs)与人胰腺突触角蛋白6(STX6)在细胞内是否存在相互作用,为进一步研究HBV影响糖、脂代谢机制奠定研究基础。方法构建真核表达质粒pACT-STX6,采用哺乳动物双杂交技术验证LHBs与STX6之间的相互作用。结果实验组和各阴性对照组相对荧光素酶活性值均存在差异,且均具有显著统计学意义(P<0.01)。结论 LHBs与人胰腺STX6在胰腺细胞内存在相互作用。Objective To confirm the intra-cellular interaction between HBV large surface protein （LHBs） and homo sapiens syntaxin 6 protein （STX6）, which brought some new clues for studying on the molecular biology mechanism of glucose and lipid. Methods Eukaryotic expression vector pACT-STX6 and confirmed the interaction between LHBs and homo sapiens syntaxin protein through mammalian two-hybrid experiment. Results The research constructed eukaryotic expression vector pACT-STX6. Mammalian two-hybrid experiment showed that there were significant differences in the relative luciferase activity value between experimental group and each negative control group were（P 0.05）. Conclusions LHBs might be interacted with homo sapiens syntaxin 6 protein in cells.

关 键 词：HBV表面抗原大蛋白 突触角蛋白6 哺乳动物双杂交 

分 类 号：R392.1[医药卫生—免疫学]