草莓FaSig转录因子基因的克隆及序列分析  

Cloning and Characterization of FaSig Gene in Strawberry

作　　者：余倩 沈元月[1] 

机构地区：[1]农业应用新技术北京市重点实验室,北京农学院植物科学技术学院,北京102206

出　　处：《北京农学院学报》2012年第2期21-23,共3页Journal of Beijing University of Agriculture

基　　金：国家自然基金资助项目(30971977);北京市自然科学基金资助项目(6082005);北京市教委联合资助重点项目(KZ200910020001)

摘　　要：Sigma因子作为一个转录正调控因子参与微生物糖代谢的调控。为了研究果实中Sigma因子的功能,利用RT-PCR与RACE扩增方法,从草莓果实cDNA中分离出一个草莓Sigma转录因子cDNA全长,进行测序和序列分析。结果表明,克隆的cDNA片段全长为2 008bp,基因内部含有完整的开放阅读框架,可编码长度为520个氨基酸残基的蛋白质,所推导的蛋白质氨基酸序列与葡萄(XM002268673)、大豆(XM003544709)、苜蓿(XM003602150)Sigma转录因子基因分别具有78%,77%和77%的氨基酸序列同源性,并含有典型的HTH结构域,表明草莓果实中Sigma属于σ70家族。Sigma factor, serving as a positive transcription factor, is involved in the regulation of microbial sugar metabolism. In order to study the function of sigma factors in fruit, the experimenti solated the full-length cDNA encoding Sigma factor from strawberry by reverse transcription polymerse chain reaction（RT-PCR）and rapid-amplification of cDNA ends（RACE）. The results showed that Sigma factor was 2008bp,containing a open reading frame（ORF）which encoded a protein of 520 amino acids with 78% ,77% and 77% simi- larity in amino acid sequence conmpared with those encoded by Sigma in Vitis vinifera, Glycine max and Medicago truncatula, respective- ly, and contained a typical HTH domain, strawberry fruit Sigma belonged to the σ^70 family.

关 键 词：草莓 sigma转录因子 基因克隆 

分 类 号：S668.4[农业科学—果树学]