胆酸钠多克隆抗体的制备及其快速检测方法研究  

The Study on the Preparation of Sodium Cholate Polyclonal Antibody and its Fast Determination Method

作　　者：陈江源[1] 刘洁[2] 蔡建中[1] 付云洁[3] 刘志国[3] 

机构地区：[1]江汉大学卫生技术学院,湖北武汉430016 [2]江汉大学文理学院,湖北武汉430056 [3]武汉工业学院生物与制药工程学院,湖北武汉430023

出　　处：《江汉大学学报（自然科学版）》2012年第2期77-81,共5页Journal of Jianghan University：Natural Science Edition

摘　　要：目的:探索用胶体金免疫层析法快速检测胆酸钠含量。方法:用碳二亚胺法合成的免疫抗原胆酸钠-BSA,免疫新西兰白兔,制备抗胆酸钠多克隆抗体。用酶联免疫吸附法(ELISA)鉴定特异性抗体效价。制备胆酸钠—OVA抗原—胶体金复合物,组装检测试纸。结果:胆酸钠抗体的效价为1∶80 000,建立的胶体金快速测定法对胆酸钠的检测限为12μg/mL。结论:试纸条法是快速检测胆汁酸含量的有效方式,在临床检测方面具有广阔的运用前景。Objective：To develop colloidal gold immunochromatographic（IC） method for rapidly detecting the concentration of Sodium Cholate.Methods： Sodium Cholate-BSA immunogen was obtained via carbodiimide method.Then the immunoantigen was injected into New Zealand rabbits to prepare the anti-sodium cholate polyclona antibody according to routine immunity procedure.The specific antibody of Bile acid natrium was identified by indirect enzyme linked immunosorbent assay（ELISA）.Results： The sodium cholate-OVA antigen-colloidal gold compounds were made for test strip preparation.It was proved that the specificity of the antibody was 1 ∶ 80 000,and the detectability was 12 μg/mL by the method.Conclusion： Accordingly,IC test strip method is effective and sensitive for rapidly detecting sodium cholate,which will probably have a broad clinical application prospect.

关 键 词：胆酸钠 多克隆抗体 ELISA 胶体金 试纸条 

分 类 号：R392.7[医药卫生—免疫学]