基质金属蛋白酶及其抑制剂在川崎病患儿冠脉损害中的临床意义  

The significance of MMPs and TIMPS with coronary artery lesions in Kawasaki disease

作　　者：陈书琴[1] 朱红枫[1] 杜娟[1] 

机构地区：[1]泸州医学院附属医院儿科,四川泸州646000

出　　处：《西部医学》2012年第5期914-915,917,共3页Medical Journal of West China

摘　　要：目的研究川崎病(KD)患儿血清基质金属蛋白酶3(MMP-3)、基质金属蛋白酶抑制剂1(TIMP-1)的表达水平,探讨其在KD冠脉损害中的临床意义。方法研究组为30例川崎病患儿,冠脉损害(CAL)组15例,冠脉正常(NCAL)组15例,分别收集急性期、恢复期外周血制备血清。对照组为30例年龄相仿的正常健康儿童。用酶联免疫吸附实验(ELISA)测定各组患儿血清中MMP-3、TIMP-1的水平。结果川崎病CAL组血清MMP-3、MMP-3/TIMP-1和NCAL组相比明显升高(P<0.05);CAL组血清TIMP-1水平较NCAL组明显降低(P<0.01)。KD急性期MMP-3、TIMP-1、MMP-3/TIMP-1与恢复期和正常对照组相比较均明显升高(P<0.01)。结论川崎病CAL组血清MMP-3和NCAL组相比明显升高,提示MMP-3可以作为预测与早期诊断CAL的客观指标。KD急性期MMP-3、TIMP-1、MMP-3/TIMP-1水平明显高于恢复期和对照组,提示MMP-3、TIMP-1、MMP-3/TIMP-1可作为早期诊断川崎病的一个客观生化指标。Objective To explore the expression of MMP-3 and TIMP-1 in Kawasaki disease and discuss their sig-nificance in the coronary artery lesion（CAL）with KD. Methods The serum was collected from 30 KD children （15 pa-tients with coronary arterial lesions, group CAL; and 15 patients with no coronary arterial lesions, group NCAL. ） Serum collection with KD in the acute phase and convalescence phase. Control group：30 normal children with the similar age. The levels of MMP-3, TIMP-1, were detected by Enzyme-linked immunosorbent assay （ ELISA ） in serum. Results MMP-3 and MMP-3/TIMP-1 of group CAL are compared with group NCAL, were significantly higher than that in group NCAL（P〈0.05）. TIMP-1 of group NCAL is compared with group CAL,is significantly higher than that in group CAL （P〈0.01） ; acute phase and convalescence phase MMP-3,TIMP-land the ratio of MMP-3/TIMP-1 level are compared with Normal control group are significantly higher than that in group normal（P〈0.01）. Conclusion MMP-3 of group CAL is significiantly higher than group NCAL, Therefore, the measurement of serum MMP-3 might be of important clini- cal value in the prediction and the early diagnosis of KD with coronary artery lesion. In acute stage serum MMP-3 and TIMP-1 levels and the ration of MMP-3/TIMP-1 were higher in patients with KD than that in convalescent stage and con- trol group; suggesting that these data are important objective markers with diagnosis KD in early stage.

关 键 词：基质金属蛋白酶 基质金属蛋白酶抑制剂 川崎病 冠脉损害 

分 类 号：R725.5[医药卫生—儿科]