花曲柳体胚发生和植株再生  

Somatic Embryogenesis and Plantlet Regeneration of Fraxinus rhynchophylla

作　　者：杨玲[1] 单琳[1] 沈海龙[1] 祁永会 

机构地区：[1]东北林业大学林学院林木遗传育种国家重点实验室,哈尔滨150040 [2]黑龙江省林业科学研究所,哈尔滨150040

出　　处：《林业科学》2012年第4期49-55,共7页Scientia Silvae Sinicae

基　　金：黑龙江省科技攻关重大项目课题(GA09B202-06);中央高校基本科研业务费专项资金(DL10BA04);黑龙江省博士后资助经费(LBH-10284);中国博士后科学基金(20110491015)

摘　　要：以花曲柳合子胚的单片子叶为外植体成功诱导出体胚并获得再生植株。未成熟合子胚的子叶在添加400mg·L-1水解酪蛋白、0.25mg·L-16-BA、1.5mg·L-1NAA、70g.L-1蔗糖和6g·L-1琼脂的MS1/2培养基上可以成功诱导产生体胚,诱导率达到34.7%,每个外植体上体胚数量为2～9个。成熟合子胚的子叶在添加0.25mg·L-16-BA、2mg·L-1NAA的MS1/2培养基(其他成分同上)上可以成功诱导产生体胚,诱导率为10.0%。体胚在MS1/2培养基上经过成熟培养后可以正常萌发,萌发率87.6%。萌发的体胚植株在MS1/2+0.01mg·L-1NAA培养基上生长较好,具备实生幼苗的外观特征。经炼苗后的体胚苗移植到栽培基质(草炭土:蛭石:珍珠岩体积比为5:4:1)中可以正常生长,成活率为75.0%。Somatic embryogenesis and plant regeneration of Fraxinus rhynchophylla were obtained by using single cotyledons of zygotic embryos as explants.Cotyledons of immature zygotic embryos,cultured on half strength MS medium supplemented with 400 mg·L^-1 hydrolyzed casein,70 g·L^-1 sugar,6 g·L^-1 agar,0.25 mg·L^-1 6-BA and 1.5 mg·L^-1 NAA,were able to be successfully induced somatic embryos.The somatic embryo（SE） induction rate could reach 34.7% and about 2-9 SEs were produced per each explant.However,cotyledons from mature zygotic embryos,culture on a similar medium with modification of adding extra 0.5 mg·L^-1 NAA,and the SE induction rate could reach 10.0%.Somatic embryos after in vitro maturation on a MS1/2 medium could normally germinate,and the germination percentage rate reached to 87.6%.The germinated somatic plantlets grew well on MS1/2 medium containing 0.01 mg·L^-1 NAA and had the similar morphological characteristics of zygotic seedlings.Acclimated somatic plantlets grew normally after transplanted in a medium with 50% peat moss,40% vermiculite and 10% polite,with 75.0% survival rate.

关 键 词：花曲柳 组织培养 体细胞胚胎发生 植株再生 

分 类 号：S718.46[农业科学—林学] Q943.1[生物学—植物学]