BAM8-22对吗啡耐受的影响  

Effect of Bovine Adrenal Medulla 8-22 on Morphine Tolerance in Rats

作　　者：江剑平[1] 洪炎国[1] 

机构地区：[1]福建师范大学生命科学学院,福建省发育和神经生物学重点实验室,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2012年第3期101-105,共5页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(30970985);福建省教育厅资助项目(JK2010014);福建师范大学优秀青年骨干教师培养基金资助项目(2008100238)

摘　　要：采用缩足反射观察椎管内注射感觉神经元特异性受体(SNSR)激动剂牛肾上腺髓质8-22肽(BAM8-22)对吗啡耐受的影响.结果表明,椎管内注射BAM8-22(0.1nmol)后,吗啡抗伤害作用的半数有效剂量由耐受时的80.99μg恢复至12.38μg(P<0.001);隔天混合给予BAM8-22后,能显著延缓吗啡耐受,第6天吗啡抗伤害作用的半数有效剂量为14.84μg,与吗啡耐受组相比有极显著差异(P<0.001);但这两组动物吗啡的抗伤害作用没有达到正常吗啡组水平(P<0.05和P<0.01).连续椎管内单独注射BAM8-22 6d后,吗啡抗伤害作用的半数有效剂量为12.23μg,明显高于正常吗啡组(P<0.01),但又明显低于吗啡耐受组(P<0.001).而椎管内连续6d混合给予BAM8-22和吗啡,并不能阻止吗啡耐受.研究表明,椎管内注射BAM8-22能部分翻转或延缓吗啡的耐受,而BAM8-22的长期作用则会部分降低吗啡的抗伤害作用,提示感觉神经元特异性受体参与了阿片受体功能的调制.To investigate the effect of intrathecal （i. t.） administration of bovine adrenalmedulla 8-22 （BAM8-22）, an agonist of sensory neuron-specific receptors （SNSR）, on mor- phine tolerance by paw withdrawal test. It was found that i.t. administration of BAM8-22（0. 1 nmol） evidently reversed established morphine tolerance, half effective dose of mor- phine antinociception reduced from 80.99μg in morphine tolerance to 12.38μg Mter BAMS-22 reversed （P 〈 0. 001） . Co-administration of BAM8-22 （0. 1 nmol） every other day, but not daily, with morphine remarkably attenuated the development of morphine tolerance, halfeffective dose of morphine antinociception in sixth day was 14.84 μg （P〈0. 001） . But antinociception of these two groups didn＇t reach to morphine in naive group level （P〈0.05and P〈0. 01） . Half effective dose of morphine antinociception was 12.23 μg after i.t. ad- ministration of BAM8-22 （0. 1 nmol） daily for six days, it was significantly higher than mor-phine in naive group （P ,（0.05） , but evidently lower than morphine tolerant group （P〈0. 001） . Co-administration of BAMS-22 daily, with morphine didn＇t attenuate the develop-ment of morphine to lerance. The present study showed that BAM8-22 inhibited the develop-ment and expression of tolerance to morphine, while chronic BAM8-22 weakened antinoci- ceptive response of morphine. These results suggest that SNSR is involved in the modulation of opioid receptors function.

关 键 词：牛肾上腺髓质8-22肽 吗啡耐受 半数有效剂量 感觉神经元特异性受体 

分 类 号：R338.2[医药卫生—人体生理学]