斑鳜myostatin基因及其启动子的克隆与序列分析  

Cloning and Sequence Analysis of the Myostatin Gene and its Promoter in Golden Mandarin Fish(Siniperca scherzeri)

作　　者：孙科军[1,2] 刘希良[2] 王开卓[2] 陈敦学[2] 褚武英[2] 陈韬[1] 张建社[2] 成嘉 

机构地区：[1]湖南农业大学动物医学院,长沙410128 [2]长沙学院生物工程与环境科学系,长沙410003

出　　处：《基因组学与应用生物学》2012年第2期133-140,共8页Genomics and Applied Biology

基　　金：湖南省自然科学基金(11JJ3034和09JJ6037)资助

摘　　要：采用Tail-PCR和常规PCR技术首次克隆出斑鳜myostatin基因及其启动子序列。经生物信息学分析发现,斑鳜myostatin基因由3个外显子和2个内含子组成,编码区1131bp,共编码376个氨基酸。斑鳜myostatin启动子区域大小为840bp,存在1个TATAA-box、4个E-box和1个CAAT-box作用元件。利用软件CLUSTALW和MEGA3.1构建14种硬骨鱼的myostatin启动子的系统进化树结果表明:斑鳜同大口黑鲈亲缘关系最近,与鲤鱼和缨野鲮亲缘关系较远,其结果与传统形态学分类中的亲缘关系一致。斑鳜myostatin基因及其启动子克隆与特征分析,将为进一步研究鱼类myostatin基因的表达调控及其功能分析提供参考。The sequences of myostatin gene and its promoter in Golden Mandarin Fish（Siniperca scherzeri）,for the first time,were cloned through the conventional PCR and Tail-PCR technology.Bioinformatics analysis shows that the myostatin gene consists of three exons and two introns and the coding region is 1 131 bp encoding 376 amino acid residues.The nucleotide sequence analysis shows that myostatin promoter is 840 bp and contains several regulatory elements,such as one TATAA-box,four E-boxes and one CAAT-box.The phylogenetic tree of the myostatin gene promoters from 14 teleost species was constructed with CLUSTALW and MEGA3.1.The result indicates that Golden Mandarin Fish（Siniperca scherzeri） has a closer phylogenetic relationship with Largemouth bass（Micropterus salmoides）,but it is far from Cyprinus carpio and Labeo fimbriatus.This result is consistent with general morphological taxonomy.This study would contribute to the further research on the regulation of myostatin expression and the function of myostatin in teleost fish.

关 键 词：斑鳜 肌肉生长抑制素 启动子 序列分析 

分 类 号：Q75[生物学—分子生物学] S917.4[农业科学—水产科学]