一步法PCR联合荧光原位杂交检测曲霉菌属感染标本  

One-step PCR combined with fluorescence in situ hybridization detection of specimens infected with Aspergillus

作　　者：潘峰[1] 周东风[2] 肖晶[2] 何世斌[3] 孟浦[2] 李立家[3] 

机构地区：[1]华中科技大学同济医学院附属协和医院放射科,湖北武汉430022 [2]华中科技大学同济医学院附属协和医院儿科,湖北武汉430022 [3]武汉大学生命科学学院植物分子细胞遗传学室,湖北武汉430072

出　　处：《中华医院感染学杂志》2012年第10期2223-2226,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金项目(No.30771204)

摘　　要：目的联合新颖的一步法PCR检测法和荧光原位杂交(FISH)检测法,检测病理诊断曲霉菌属感染标本。方法收集病理诊断曲霉菌属感染标本33份作为试验组;随机收集非曲霉菌属感染标本12份作为阴性对照组;采用标准黑曲霉菌株作为阳性对照组;建立新颖的一步法PCR检测技术,联合FISH检测法对试验组及对照组标本进行检测。结果一步法PCR可以快速、特异地检测量仅0.01 g的黑曲霉菌丝;试验组中,有16份标本两种检测均为阳性,12份检测均为阴性;所有阴性对照组标本两种检测均为阴性。结论一步法PCR检测曲霉菌属具有很高的检测灵敏性和特异性,其联合FISH检测,可作为一种有潜力的曲霉菌属快速检测方法。OBJECTIVE To detect the specimens infected with Aspergillus by novel one-step PCR combined with fluorescence in situ hybridization（FISH）.METHODS A total of 33 specimens with pathologically suspected Aspergillus infections were collected as experimental group;12 specimens without Aspergillus infection were randomly collected as the negative control group;a standard Aspergillus niger was collected as a positive control group;a novel one-step PCR method was established to combine with FISH to detect the specimens in the experimental group and the control group.RESULTS The one-step PCR could rapidly and specifically detect the Aspergillus niger with only 0.01g mycelia;totally 16 specimens in experimental groups were detected positive with both one step PCR and FISH,12 specimens detected negative;the specimens in the negative control group were detected negative.CONCLUSION One-step PCR is with high sensitivity and specificity in the detection of Aspergillus,and its combination with FISH can serve as an potential rapid detection of Aspergillus.

关 键 词：曲霉菌属 侵袭性真菌感染 荧光原位杂交 聚合酶链反应 

分 类 号：R446[医药卫生—诊断学]