云南省麻疹野病毒不同基因型的流行分布状况  

Epidemic Distribution Characteristics of Wild-type Measles Viruses Genotypes in Yunnan Province

作　　者：李立群[1] 庞颜坤[1] 王慧玲[2] 张杰[1] 彭敏[1] 余文[1] 罗梅[1] 丁峥嵘[1] 

机构地区：[1]云南省疾病预防控制中心,昆明650022 [2]中国疾病预防控制中心病毒病预防控制所世界卫生组织西太平洋区麻疹参比实验室,北京102206

出　　处：《中国疫苗和免疫》2012年第2期105-109,117,共6页Chinese Journal of Vaccines and Immunization

摘　　要：目的了解云南省麻疹野病毒不同基因型的流行分布状况。方法用B95a细胞[Epstein-Barr(EB) Virus-Transformed,Marmoset B Lymphoblastoid Cell Line;埃泼斯坦-巴尔病毒转化的绒猴淋巴母细胞]和Vero/SLAM细胞[Vero Cell Transfected to Express the Human Signaling Lymphocyte Activation Molecule(SLAM),淋巴信号激活因子转染的非洲绿猴肾细胞]从麻疹爆发和散发患者的标本中分离麻疹野病毒,用逆转录-聚合酶链反应扩增核蛋白(Nucleoprotein,N)基因羧基末端的676个核苷酸片段,对扩增产物进行核苷酸序列测定。并以N基因羧基末端450个核苷酸序列构建基因亲缘性关系树,进行核苷酸变异分析。结果云南省2004～2011年共分离和检测到46株麻疹病毒及2株病毒核酸,43株为H1基因型,分布于14个县(区)及四川省普格县;4株为d11基因型,分布于边境孟连县及缅甸的班康市;1株为A基因型,来自龙陵县。结论麻疹病毒H1基因型是云南省优势本土基因型,d11基因型是从缅甸输入后连续两年于缅甸接壤的地区发现的麻疹野病毒,A基因型为中国沪191疫苗株相似株。Objective To know the epidemic and distribution of wild-type measles viruses genotypes in Yunnan province. Methods Measles viruses were isolated from the specimens, which collected from measles outbreaks and sporadic patients by B95a cell [- Epstein-Barr （EB） Virus-Transformed,Marmoset B Lymphoblastoid Cell Line] and Vero/SLAM cell [Vero Cell Transfected to Express the Human Signaling Lymphocyte Activation Molecule （SLAM）]. Fragment of 676 nucleotide acids of the carboxyl end of nucleoprotein gene were amplified by reverse transcription-polymerase chain reaction （RT-PCR） and then the PCR products were directly sequenced and analyzed. Besides, phylogenetic tree was constructed based on 450 nucleotide acids of the carboxyl end of nucleoprotein gene, and homological analysis was performed. Results 46 wild-type measles viruses and 2 nucleotide acids were isolated in Yunnan province from 2004 to 2011.43 strains were identified as Ht genotype, distributed in 14 counties and Puge county, Sichuan province. 4 strains were identified as d11 genotype, distributed in Menglian county, Yunnan province and Bankang county, Myanmar. 1 strain was identified as A genotype, from Longling county. Conclusion H1 genotype was predominant genotype of wild measles viruses circulated in Yunnan province, d11 genotype currently has circulated in the border areas after imported from Myanmar. A genotype was the same with Chinese measles vaccine strain of Shanghai191.

关 键 词：麻疹病毒 流行分布 H1基因型 d11基因型 

分 类 号：R373.11[医药卫生—病原生物学]