紫外分光光度法测定吴茱萸碱-β-环糊精的包合率  

Determination of Inclusion Rate of Evodiamine-β-Cyclodextrin by UV Spectrophotometry

作　　者：刘宏明[1] 谭群友[2] 柳珊[1] 邓波[2] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心和生物化学与分子药理学重点实验室,重庆市渝中区医学院路1号400016 [2]第三军医大学大坪医院野战外科研究所胸外科中心,重庆市渝中区长江支路10号400042

出　　处：《光谱实验室》2012年第3期1334-1337,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：教育部博士点基金资助项目(20095503120008);重庆市教育委员会资助项目(首批高等学校优秀人才资助;KJ090308)

摘　　要：建立吴茱萸碱-β-环糊精的包合率测定方法。采用紫外分光光度法测定吴茱萸碱-β-环糊精的包合物中吴茱萸碱的含量,由此计算包合率。结果表明吴茱萸碱在225nm处有最大吸收,浓度在1.03—4.12μg.mL-1时与吸光度呈良好的线性关系,回归方程为:A=0.1661C+0.0145,r=0.9997(n=7);平均回收率为99.06%,RSD为0.61%。本法操作简便易行,具有良好的重现性和精密度。The method for determination of the inclusion rate of evodiamine-β-cyclodextrin was established.The content of evodiamine in evodiamine-β-cyclodextrin clathrate compound was determined by UV spectrophotometry,then the inclusion rate was calculated on the base.The evodiamine had the maximum absorption at wavelength of 225nm,and there was the good linear relation between absorbance and concentration in the range of 1.03—4.12μg·mL-1.The regression equation was A=0.1661C＋0.0145 with correlation coefficient of 0.9997（n=7）.The average recovery was 99.06% with RSD of 0.61%.The method is easy and simple operation with good repeatability and precision.

关 键 词：吴茱萸碱 包合物 包合率 

分 类 号：O657.32[理学—分析化学]