孔雀石绿-锌(Ⅱ)-牛血清白蛋白的共振光散射及其分析应用  

Resonance Light Scattering of Malachite Green-Zn(Ⅱ)-Bovine Serum Albumin and Its Analytical Application

作　　者：宋林[1] 杲秀珍[1] 朱成财[2] 李武[3] 郭海燕[1] 冯瑛[1] 郑红[1] 

机构地区：[1]青海师范大学化学系,西宁市五四西路38号810008 [2]中国科学院青海盐湖研究,所西宁市新宁路18号810008 [3]中国科学院青海盐湖研究所,西宁市新宁路18号810008

出　　处：《光谱实验室》2012年第3期1341-1344,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：教育部科学技术研究重点项目(208155);青海省科技厅项目(2010-Z-716)

摘　　要：基于牛血清白蛋白(BSA)使孔雀石绿(MG)-锌(Ⅱ)配合物体系的共振光散射光谱(RLS)信号增强,建立了一种测定痕量蛋白质的新方法。探讨了pH、MG浓度、金属离子浓度、共存物质等因素对共振光散射强度的影响。在优化实验条件下,RLS强度与BSA浓度的线性范围为0.1—25μg/mL,检出限0.06μg/mL,运用该方法对合成样品加标回收测定,结果满意。A novel method for the determination of trace protein was established based on the enhancement of resonance light scattering（RLS）intensity in malachite green-Zn（Ⅱ） compound system that caused by BSA.The influences of the concentration of MG and metallic ion,pH,substance on the intensity of RLS were discussed in detail.Under optimization of experiment conditions,the intensity of RLS for the system was proportional to the concentration of bovine serum albumin in the range of 0.1—25μg/mL and the detection limit was 0.06μg/mL.This method was used for the determination of spiked recovery for the synthetic samples with the satisfactory result.

关 键 词：孔雀石绿-锌(Ⅱ) 牛血清白蛋白 共振光散射 光谱分析 

分 类 号：O657.32[理学—分析化学]