固相萃取-高效液相色谱测定污水中的地塞米松  

Determination of Dexamethasone in Sewage by SPE-HPLC

作　　者：何东平[1] 龙友琦[1] 韩美娜[1] 尚京川[1] 

机构地区：[1]重庆医科大学药学院,重庆市渝中区医学院路1号400016

出　　处：《光谱实验室》2012年第3期1469-1472,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：建立固相萃取-高效液相色谱法测定污水中地塞米松含量的方法。水样经固相萃取柱萃取富集后进行HPLC分析,以甲醇:水=65:35(V/V)为流动相,色谱柱为Agilent C18柱(5μm,4.6mm×150mm),柱温30℃;流速为1mL/min;紫外检测波长为240nm。地塞米松在25—300ng/mL范围内线性关系良好(n=5,R2=0.9992),最低检测浓度为25ng/mL(S/N>10),回收率为98.36%,地塞米松的低、中、高的日内精密度与日间精密度RSD均小于3%。本法准确、灵敏,能较好的应用于污水中地塞米松的测定。The method for the determination of the content of dexamethasone in sewage by solid phase extraction-high performance liquid chromatography（SPE-HPLC） was established.The water samples were enriched by solid phase extraction column,then determined by HPLC on a Agilent C18（5μm,4.6mm×150mm） chromatographic column with mobile phase consisting of a mixture of methanol and water（65∶35,V/V） at a flow rate of 1mL/min.The ultraviolet detection wavelength was 240nm,and the column temperature was 30℃.There was the good linear relation for the concentration of dexamethasone in the range of 25—300ng/mL（n=5,R2=0.9992）,and the limit of quantitation（LOQ,S/N10） was found to be 25ng/mL（S/N10）.The recovery was 98.36%.The relative standard deviation（RSD） of the intra-day and the inter-day were both less than 3%.This method is accurate and sensitive,that can be applied to determining dexamethasone in sewage.

关 键 词：高效液相色谱 地塞米松 固相萃取 污水 

分 类 号：O657.72[理学—分析化学]