铽-茜素红作为光散射探针测定洛美沙星的共振瑞利散射光谱及其应用  

Resonance Rayleigh Scattering Spectrum in Determination of Lomefloxacin with Tb^(3+)-Alizarin Red as Light Scattering Probe and Its Application

作　　者：王媚[1] 韩权[2] 杨晓慧[2] 

机构地区：[1]陕西中医学院药学院,陕西省咸阳市世纪大道中段712046 [2]西安文理学院化学与化学工程系,西安市太白南路168号710065

出　　处：《光谱实验室》2012年第3期1477-1481,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：西安市科技计划项目(CXY09015-5)

摘　　要：提出了共振瑞利散射光谱法测定洛美沙星的新方法。在pH 5.0—6.0的HAc-NaAc缓冲溶液中,铽()与洛美沙星(Lomefloxacin,LOM)抗生素能形成阳离子配合物,它可进一步通过静电引力和疏水作用与茜素红(Alizarin Red,AR)阴离子反应形成1:2:1(Tb3+:LOM:AR)三元离子缔合物,此时将出现新的共振瑞利散射(Resonance Rayleigh Scattering,RRS)光谱,其两个散射峰分别位于370nm和590nm处。在370nm处,一定浓度的洛美沙星与散射增强(ΔI)成正比,其线性范围和检出限(3σ)分别是0.004—3.52μg.mL-1和14.3 ng.mL-1。该方法简单、快速、具有良好的选择性和重复性,可用于不同体液中洛美沙星药物的测定。文中还对反应机理作了讨论。A novel method for the determination of lomefloxacin（LOM） by resonance Rayleigh scattering（RRS）spectrometry was developed.In pH 5.0—6.0 HAc-NaAc buffer solution,lomefloxacin can react with Tb3＋ to form cation chelates,which further bond with alizarin red（AR） anion to form ternary ion-association complexes（Tb3＋∶LOM∶AR） at the ratio of 1∶2∶1 by electrostatic attraction and hydrophobic interaction,that resulted in a new RRS spectrum.The two scattering peaks located at 370nm and 590nm.The RRS intensity enhancement（ΔI） was proportional to the certain concentration of LOM in the range of 0.004—3.52μg·mL-1,and the detection limit was 14.3ng·mL-1.The method is simple,rapid with the good selectivity and repeatability,that is applied to the determination of LOM in the different body fluid.The reaction mechanism was also investigated.

关 键 词：洛美沙星 铽(Ⅲ) 茜素红 共振瑞利散射 

分 类 号：O657.32[理学—分析化学]