绞股蓝种子中α-桐酸的定性和定量分析  

Qualitative and Quantitative Analysis of Alpha-Eleostearic Acid in G.Pentaphyllum Seeds

作　　者：姜东亮[1] 马彩霞[1] 肖娅萍[1] 

机构地区：[1]陕西师范大学生命科学学院,西安市长安南路199号710062

出　　处：《光谱实验室》2012年第3期1848-1853,共6页Chinese Journal of Spectroscopy Laboratory

基　　金：国家"十五"科技攻关项目(2001BA701A35)

摘　　要：用RTX-5MS弹性石英毛细管柱(0.25mm×30m×0.25μm)气相色谱-质谱法(GC-MS)分析绞股蓝种子中的α-桐酸。用相对保留时间和质谱对α-桐酸进行定性分析,用内标法对α-桐酸进行定量分析。结果表明,绞股蓝种子中含有α-桐酸,在0.34—1.37mg/mL范围内线性关系良好(R2=0.9996),平均回收率为99.91%,且绞股蓝种子中α-桐酸的含量为66.9mg/g。实验结果为以后的绞股蓝种子中α-桐酸的开发与利用提供理论依据。A gas chromatography-mass spectrometry（GC-MS） was established to identify composition and determine the content of alpha-eleostearic acid in G.Pentaphyllum seeds,with RTX-5MS fused-silica capillary column（0.25mm×30m×0.25μm）.The composition of alpha-eleostearic acid was qualitatively identified through the relative retention time and mass spectrometry,alpha-eleostearic acid was quantitatively determined by internal standard method.The results revealed that alpha-eleostearic acid was detected and the content was high.The determination of alpha-eleostearic acid using GC-MS was expressed to be A=2.7924C-0.4851 with a correlation coefficient of 0.9996.An excellent linear range for alpha-eleostearic acid was 0.34—1.37mg/mL.The average recovery was 99.91%.Total content of alpha-eleostearic acid in G.pentaphyllum seeds was 66.9mg/g.The high yield suggests an effective way for sustainable production of alpha-eleostearic acid.

关 键 词：绞股蓝种子 α-桐酸 内标法 气相色谱-质谱法 

分 类 号：O657.63[理学—分析化学]