实验性大鼠液压冲击脑损伤神经功能评分与脑水肿、水通道蛋白4表达的关系  

The relationship between the change of aquaporin4, brain edema and neuronal function score of fluid percussion brain injury in rats

作　　者：孙国柱[1] 赵宗茂[1] 杨雷芳[1] 刘性强[1] 王目刚[1] 韩仰军[1] 

机构地区：[1]河北医科大学第二医院神经外科,石家庄050000

出　　处：《中华行为医学与脑科学杂志》2012年第5期392-394,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：河北省自然基金资助项目（C2008001084）;河北省科技厅资助项目（08206123D）;河北省医学科学重点课题（20090091）

摘　　要：目的观察液压冲击脑损伤大鼠神经功能评分，及其与脑水肿和水通道蛋白4（AQP4）高表达的关系。方法制作SD大鼠液压冲击脑损伤模型并对动物进行神经功能评分，应用干湿重法测定脑组织含水量，免疫组织化学染色方法和免疫蛋白印迹检测AQP4表达变化。结果TBI组动物神经功能评分12h出现下降[（11．17±1．32）分]，24h下降至最低值[（10．17±0．75）分]，3d逐渐升高[（10．66±1．37）分]；脑含水量12h明显增加[（80．27±1．47）％]，24h达到最高[（82．19±0．97）％]，持续至3d开始下降[（81．74±1．69）％]；WesternBlot结果显示AQP4表达于12h明显增高（OD：0．65±0．05），24h达到最高值（OD：0．72±0．08），3d下降（OD：0．56±0．07），7d（OD：0．49±0．09）仍高于对照组（OD：0．15±0．05）；免疫组化显示AQP4表达趋势与Western Blot结果一致。线性相关分析发现神经功能评分与脑含水量之间存在负相关性（r=-0．615，P〈0．01）；神经功能评分与AQP4蛋白表达之间也呈负相关（r=-0．605，P〈0．05）。结论液压冲击伤大鼠的神经功能评分降低，其与脑水肿和AQP4表达呈负相关，可能与AQP4通过介导脑水肿的发生、间接参与神经功能损伤有关。Objective To observe the relationship between neuronal function score, brain edema and aquaporin4 （AQP4） expression of fluid percussion brain injury in rats. Methods The fluid percussion models of brain injury of rats were established by using the improved device. Nervous function score, brain water content, histological changes, AQP4 expression were observed by Shapira and Wahld method, dry-wet measure,light microsco- py,immunohistochemistry and western blot at 1 h, 6 h, 12 h, 24 h, 3 d and 7 d after operation respectively. Results Nervous function score in TBI group decreased at 12h（ 11.17 ± 1.32） ,reached its minimum at 24h（ 10.17 ±0.75） ,and recoved partially at 3rd day（ 10.66 ± 1.37）. The water content obviusly increased in those of TBI group at 12 h （ （ 80.27 ± 1.47 ） % ）, reached its peak at 24 h （ （ 82.19 ± 0.97 ） % ）, and then began to drop at 3 d （ （81.74 ± 1.69）% ）, while Western blot showed that AQP4 immunoreactive expression obviusly increased at 12 h （OD：0.65 ±0.05） ,reached its maximum at 24h（OD：0.72 ±0.08） ,and decreased at 3 d（OD：0.56 ±0.07） , and immunohistochemistry showed the same trendency of AQP4 expression as that of Western blot. The linear regression analysis indicated that nervous function score had a negtive correlation with expression of AQP4 in edematous brain and change of brain water content respectively（ r = -0.615, P 〈 0.01; r = -0.605, P 〈 0.05）. Conclusion Nervous function score of fluid percussion brain injury in rats decrease, has negative relationship with brain edema and AQP4 expression, and possible mechanisms is that AQP4 is indirectly involed in nerve function impairment by mediating brain edema.

关 键 词：脑损伤 液压冲击 脑水肿 水通道蛋白4 

分 类 号：R651.1[医药卫生—外科学]