条石鲷视觉器官早期发育的组织学观察  

Histological observation of eye ontogeny in rock bream larvae (Oplegnathus fasciatus)

作　　者：何滔[1,2] 肖志忠[1] 刘清华[1] 马道远[1] 徐世宏[1] 肖永双[1] 李军[1] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]西南大学动物科技学院,重庆北碚400715

出　　处：《海洋科学》2012年第3期49-53,共5页Marine Sciences

基　　金：中国科学院创新性前沿项目(KSCX2-YW-N-47-08);国家农业科技成果转化资金项目(05EFN2166000453);青岛市科技发展计划项目(05-1-HY-79)

摘　　要：应用石蜡切片技术和显微测量法,对条石鲷(Oplegnathus fasciatus)视觉器官的早期发生、分化和形成过程进行了连续观察。结果显示:在水温24℃±1.0℃的条件下,受精后10.5 h,视杯出现;受精后15.5 h,晶状体形成;受精后22 h,在脉络膜裂口处出现角膜;2日龄,巩膜和脉络膜形成;3日龄,虹膜出现;6日龄,视网膜分化完全,由色素层、视锥视杆层、外界膜、外核层、外网状层、内核层、内网状层、神经节细胞层、神经纤维层和内界膜组成;13日龄,角膜分化完全,由外向内依次为复层扁平上皮、前弹性膜、纤维层、后弹性膜和内皮层;21日龄,虹膜完全分化,由内向外依次为色素层、后缘层、基质层、前缘层和内皮层。此时条石鲷眼的各个部分(巩膜、脉络膜、视网膜、晶状体、虹膜、角膜)均发育完善。Based on the histological observation under optical microscope, we studied the ontogeny of eyes in rock bream. The results showed that at 24℃±1.0℃, the optic vesicle appeared at 10.5 h after fertilization, and the lens was visible at 15.5 h after fertilization. As the choroid fissures widened, the cornea was developed in the site at 22 h after fertilization. At 2 DAH （days after hatching）, the choroid and sclera were formed, and the iris was present at 3 DAH. At 6 DAH, the retina was well developed and consisted of pigment epithelium, rods and cones layer, external limiting membrane, outer nuclear layer, outer plexiform layer, inner nuclear layer, inner plexiform layer, ganglion cell layer, nerve fibre layer and internal limiting membrane. At 13 DAH, the cornea was well differentiated, and consisted of stratified squamous epithelium, lamina elastica anterior, fibre layer, lamina elastica porterior and en- dothelium. At 21 DAH, the iris was well developed and consisted of pigment layer, iris posterior, stroma, iris anterior and endothelium. At this time, the eyes of rock bream were developed completely.

关 键 词：条石鲷(Oplegnathus fasciatus) 早期发育 眼 组织学 

分 类 号：S917.4[农业科学—水产科学]