波纹唇鱼mtDNA D-loop序列变异分析  

The variation analysis of mtDNA D-loop sequence of Cheilinus undulatus

作　　者：胡静[1] 齐兴柱[1] 尹绍武[1,2] 骆剑[1] 朱晓平[1] 祝斐[1] 胡亚丽[2] 

机构地区：[1]海南大学海洋学院,海南大学热带生物资源教育部重点实验室,海南海口570228 [2]南京师范大学生命科学学院,江苏南京210046

出　　处：《海洋科学》2012年第4期50-56,共7页Marine Sciences

基　　金：国家自然科学基金项目(40966003);海南省自然科学基金(809009);海南省热带水生生物技术重点实验室开放基金(shkyjj0801);海南大学211工程研究生创新平台课题资助

摘　　要：为研究波纹唇鱼(Cheilinus undulatus)线粒体D-loop序列遗传多样性,作者采用聚合酶链式反应和克隆技术对海波纹唇鱼群体(n=25)的mtDNA D-loop序列进行克隆和测序,获得长度大约为1 400 bp的产物。用ClustalX软件进行排序比较,将结果导入MEGA5.0,结果显示出在这25尾个体中,共检测出66个变异位点,包括0个碱基缺失,4个碱基插入,59转换位点,2颠换位点及1个转换和颠换同时存在的位点。并用MEGA5.0软件构建该群25尾个体的NJ和UPGMA系统树。由DNASP软件计算出该波纹唇鱼群体的多态位点数(S)为62,核苷酸多样性(Pi)为0.00606,平均核苷酸差异数为6.907。结果表明波纹唇鱼群体的mtDNA-loop基因个体差异程度不明显。The aim of this paper was to study the variations in the mtDNA D-loop sequences of Cheilinus undulatus populations. Twenty-five individuals of C. undulatus were collected from the offshore area of Hainan province. One thousand and four hundreds bp nucleotide sequences of mtDNA D-loop region were obtained using PCR and clon- ing techniques. 66 variation sites including 0 deletion, 4 inserts, 59 transition sites, 2 transversion sites and l tran- sition- transversion sites were observed using ClustalX and MEGA5.0 softwares. The pairwise genetic distances of these 25 individuals were calculated using the MEGA5.0 software and based on that, the NJ phylogenetic trees was contracted for these fish populations. Analysis of this fish populations using the DNASP software showed that they had a polymorphic sites （S） of 62, a nucleotide diversity （Pi） of 0.00606 and an average number of nucleotide dif- ferences （K） of 6.907, respectively. Overall, there were no significant variations between mtDNA D-loops of C. undulatus populations.

关 键 词：波纹唇鱼(Cheilinus undulatus) MTDNA D—loop 遗传多样性 

分 类 号：Q953[生物学—动物学]