肾综合征出血热病毒对肾小管上皮细胞损伤的研究  

The study of cytopathogenic effect of hemorrhagic fever with renal syndrome virus on renal tubular cells

作　　者：姚丽君[1] 邓安国[1] 黄蓓 李元莉[1] 

机构地区：[1]同济医科大学附属协和医院肾内科,武汉430022

出　　处：《中华肾脏病杂志》2000年第3期173-176,共4页Chinese Journal of Nephrology

基　　金：卫生部资助基金!(96-1-142)

摘　　要：目的研究肾综合征出血热病毒（ＨＦＲＳＶ）对肾小管上皮细胞（ＲＴＣ）的致病作用。方法 应用ＨＦＲＳＶ感染体外培养的人胚ＲＴＣ（ＨＦＲＴＣ），采用原位分子杂交技术检测ＨＦＲＴＣ内的ＨＦＲＳＶ并作台盼蓝染色、透射电镜（ＴＥＭ）观察。结果（１）ＨＦＲＳＶ能够直接感染培养的ＨＦＲＴＣ；（２）从第７天起被感染的ＨＦＲＴＣ死亡率明显高于对照组；（３）ＴＥＭ观察表明ＨＦＲＳＶ可致ＨＦＲＴＣ的细胞膜、细胞器损害。结论ＨＦＲＳＶ能够直接损害体外培养的ＨＦＲＴＣ，其对ＲＴＣ的损害作用可能直接参予肾综出血热（ＨＦＲＳ）的发病。Objective To study the cytopathogenic effect of epidemic hemorrhagic fever with renal syndrome virus (HFRSV) on renal tubular cells(RTC). Methods Human fetal renal tubular cells (HFRTC) were cultured in vitro. HFRTC infected or not infected by HFRSV were observed by using trypan-blue stain and transmission electron microscopy(TEM). Viral-mRNA was detected by in situ molecular hybridization. Results (1) HFRSV could directly infected HFRTC: (2)The death rate of HFRTC in the infection group was significantly higher than that in the control grou 1 to 4 weeks after infection; (3) Injuries of cell membrane and cell organs after infection with HFRSV were significantly earlier and more severe as compared to control by means of TEM. Conclusion HFRSV can directly damage renal tubular cells (RTC ), which contributes to the pathogenesis of hemorrhagic fever with renal syndrome (HFRS).

关 键 词：肾综合征出血热 肾小管上皮细胞 HFRSV 

分 类 号：R512.8[医药卫生—内科学]