不同方法提取的元胡白芷产物中相关药效成分及镇痛作用的比较  

Comparison of Products from Corydalis Rhizama and Angelica Dahuricae Radix with Different Extraction Methods on Active Components and Analgesic Effects

作　　者：刘群[1] 黄欣[2] 荆俊丽[1] 苏乐群[2] 

机构地区：[1]山东大学药学院,济南250012 [2]山东省千佛山医院药学部,济南250014

出　　处：《中国现代应用药学》2012年第5期415-418,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：山东省科技攻关计划项目(2007GG2NS02053)

摘　　要：目的对二氧化碳超临界萃取(CO2-SFE)和醇回流方法所提取的元胡白芷两种不同产物进行比较。方法采用固含物量、薄层色谱(TLC)、高效液相(HPLC)和不同的大鼠疼痛模型,从相关药效成分、提取物镇痛等角度对两种提取方法所得的产物进行比较。结果 CO2-SFE法所得萃取物的量约为醇回流法的1/4,纯度为后者的6～9倍;CO2-SFE法的提取物的镇痛作用强于醇回流法,且对血中疼痛物质的影响优于醇回流法。结论在元胡白芷的提取上,CO2-SFE明显优于醇回流技术,适宜进一步推广应用。OBJECTIVE To compare the two different extracting methods：the CO2-SFE and the alcohol refluxing method, on extraction of Corydalis Rhizama and Angelica dahuricae Radix. METHODS TLC, HPLC were used to determine the extracting efficiency with the active components yield; the analgesic experiments were used to explore the differences in the analgesic effect between the two extraction methods. RESULTS CO2-SFE had 1/4 extract yield of refluxing method, but its purity was 6-9 times higher than refluxing method. It had better analgesic effect in the experimental models and had better effects on pain-related substances. CONCLUSION To extract Corydalis Rhizama and Angelica dahuricae Radix by SFE is more efficient and feasible than the traditional alcohol refluxing method.

关 键 词：元胡 白芷 二氧化碳超临界萃取 醇回流提取 

分 类 号：R284.2[医药卫生—中药学]