正交试验优化云南松松针中莽草酸的提取工艺  

Optimization of extraction process for shikimic acid from the pine needle of Pinus yunnanensis Franch by orthogonal design

作　　者：张志琴[1,2] 刘光明[1] 杨永寿[1] 肖培云[1] 

机构地区：[1]大理学院药学院,云南大理671000 [2]楚雄医药高等专科学校,云南楚雄675000

出　　处：《中国医院药学杂志》2012年第10期761-763,共3页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:30860365)

摘　　要：目的:优化云南松松针中莽草酸提取的最佳工艺。方法:采用L9(34)正交试验,以提取溶剂的浓度、溶媒用量、提取时间、提取次数为因素,HPLC法测定莽草酸含量并作为考察指标。结果:最佳提取工艺为80%乙醇为溶媒,12倍量的溶媒,回流2次,每次1.5 h。结论:优化后的提取工艺简单、稳定、提取率高。OBJECTIVE To select the optimal extraction procedure for shikimic acid from the Pine needle of Pinus yunnanensis Franch. METHODS Taking shikimic acid content as the index, L9 （3^4 ）orhogonal design method was used for optimization and HPLC for determination. The influential factors such as concentration and volume of ethanol, time of extraction and frequency of extraction. RESULTS The toptimum extraction process was as follows, 12 times of 80%alcohol, refluxing and extracting for 2 times, each time for 1.5 h. CONCLUSION This optimized process is simple, stable and efficient.

关 键 词：云南松 松针 莽草酸 正交试验 提取工艺 

分 类 号：R94[医药卫生—药剂学]