金属硫蛋白排铅和肝脏保护作用的实验研究  

Experimental study on lead excretion and liver protection of lead-exposed mice by metallothionein

作　　者：于东[1] 丁世彬[1] 廖丹[1] 蓝泽桥 李义勇 文左凤 应晨江[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院营养与食品卫生学系,湖北武汉430300 [2]武汉天峡鲟都科技有限公司,湖北武汉430060

出　　处：《工业卫生与职业病》2012年第3期150-153,共4页Industrial Health and Occupational Diseases

基　　金：国家自然科学基金(30972473)

摘　　要：目的探讨金属硫蛋白(metallothionein,MT)对染铅小鼠的排铅作用以及对肝脏的保护作用。方法小鼠连续30d饮用0.55g/L醋酸铅水溶液制作铅中毒模型组,以MT灌胃的小鼠作为治疗组,并以依地酸二钠钙作为阳性药物对照组。结果MT组小鼠股骨铅含量为(8.50±4.53)μg/g,明显低于铅模型组(P<0.05);MT组小鼠血清中丙氨酸氨基转移酶(ALT)为(27.44±3.43)U/L,明显低于铅模型组(P<0.05),但肝脏中ALT为(232.83±18.08)U/L,明显高于铅模型组(P<0.05)。MT组、正常组和铅模型组小鼠血清和肝脏中的天冬氨酸氨基转移酶(AST)比较,差异无统计学意义(P>0.05);染铅小鼠肝细胞膜Na+-K+ATP酶和Ca2+-Mg2+ATP酶活力没有明显变化。结论MT具有明显的促排铅作用;但对肝脏的保护作用不明显。Objective To study the effect of metallothionein（MT）on lead excretion and liver protection of lead exposed mice. Methods Mice successively drank 0.55 g/L lead acetate solution for 30 days to form lead -poisoned mice model. Mice administered with metallothionein were taken as MT treatment group, and CaNa2-EDTA injected mice were used as positive drug control group. Results The lead in mice femur of MT group was（8. 50±4.53） μg/g, and it was significantly lower than lead--poisoned model group （P〈 0.05）. The serum ALT in mice of MT treatment group was（27.44±3.43）U/L significantly lower than lead -poisoned model group（P〈0.05）, but the ALT in mice liver of MT treatment group was（232.83±18.08） U/L significantly higher than lead--poisoned model group（P〈0.05）. The AST in blood serum and liver of all groups showed no difference. The activities of Na＋-Kq- ATP enzyme and Caz＋ -Mgz＋ ATP enzyme in liver cellular membrane of four groups showed no significant change. Conclusions MT could significantly enhance the lead excretion, but liver protection is not so distinctive.

关 键 词：金属硫蛋白 排铅作用 肝脏保护作用 小鼠 

分 类 号：R135[医药卫生—劳动卫生]