大鼠表皮干细胞的分离及鉴定  

Isolation and Identification of Rat Epidermal Stem Cells

作　　者：郭延敏[1] 

机构地区：[1]菏泽学院制药工程系,山东菏泽274015

出　　处：《中国畜牧兽医》2012年第5期122-126,共5页China Animal Husbandry & Veterinary Medicine

基　　金：菏泽学院科研基金(XYJJKJ-8)

摘　　要：在试验中采用组织块法分离培养大鼠表皮干细胞,观察所培养的细胞形态,免疫组织化学方法检测CK15、P63、α6-integrin表达情况并用α6-integrin标记表皮干细胞经流式细胞仪检测其标记率,同时对1～6代表皮干细胞在不同培养时间计数。结果表明,采用组织块法能分离得到大鼠表皮干细胞,且获得的细胞生长良好;干细胞标记物CK15、α6-integrin、P63呈阳性。经流式细胞仪检测得知,黏附细胞被α6-integrin标记的几率为80%,而未黏附细胞被α6-integrin标记的几率仅为2%,二者相差较大。Direct-tissue culturing was used to isolate and culture rat epidermal stem cells. Then, observe the appearance of the culturing cells, and the expression of CK15, P63 and a6-integrin in ESCs were detected by immunohistochemistry staining. By the way, the ESCs marked by α6-integrin were detected by flow cytometry （FCM）. Meanwhile, the growth curve of epider- mal stem cells was also detected. We could isolate, purify and culture rat epidermal stem cells successfully by direct-tissue cul- turing method and these ceils grew well. Immuno-histochemistry staining showed that CK15, P63 and a6-integrin were strong- ly expressed in the cultured epidermal stem cells. According to the flow cytometry, the probability of sticky cells marked by α6-integrin was 80 %, while the probability of unstickv cells marked bv α6-integrin was only 2 %.

关 键 词：大鼠 表皮干细胞 分离 鉴定 

分 类 号：Q813.11[生物学—生物工程]