从羊皮肤组织中提取总RNA方法的改进  

The Improvement on the Method of Total RNA Extraction from Goat and Sheep Skin Tissues

作　　者：耿荣庆[1,2] 白丁平[1] 袁超[1] 陈玉林[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]盐城师范学院生命科学与技术学院,江苏盐城224051

出　　处：《中国畜牧兽医》2012年第5期244-247,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31101684);农业部转基因生物新品种培育科技重大专项"高产绒量转基因绒山羊新品种培育"(2011ZX08008-002)

摘　　要：在借鉴动物组织RNA提取方法的基础上,结合使用RNAlater保存液和Trizol试剂,改进了提取步骤和反应体系。利用改良的总RNA提取方法,从羊皮肤组织中快速、高效地提取了高质量总RNA,提取的总RNA可满足基于RNA分析技术的相关分子生物学试验需要。In referring to various methods for RNA extraction from different animal tissues, we modified the extraction pro cedure and reaction system by using RNAlater preservation solution and Trizol reagent. With the modified total RNA extrac- tion method, we successfully obtained high quality total RNA from goat and sheep skin tissues. Extracted total RNA could be meet the need of relevant molecular experiment based on RNA analysis technology.

关 键 词：皮肤组织 RNA提取 RNAlater保存液 

分 类 号：Q78[生物学—分子生物学]