拟南芥低温黑暗发芽基因鉴定及QTL定位  

Identification of Genes Involved in Low Temperature Germination in the Dark in Arabidopsis by Mapping Quantitative Trait Loci

作　　者：孟平红[1,2] 杨正安[1] 万发香[1] 李苍山[1] 张兴国[1] 

机构地区：[1]西南大学园艺园林学院/南方山地园艺学教育部重点实验室,重庆400715 [2]贵州省农业科学院园艺研究所,贵阳550006

出　　处：《植物遗传资源学报》2012年第3期449-455,共7页Journal of Plant Genetic Resources

基　　金：西南大学博士后基金(102060-207107);国家自然科学基金(31160238);贵州省优青人才项目(黔科合人字(2009)03号);贵州省省长基金项目(黔省专合字[2009]61号)

摘　　要：低温发芽是一个重要农艺性状,提高作物的耐寒性并确定控制低温发芽的基因是一个全球性的重大育种课题。通过对300多份拟南芥种子进行发芽试验,鉴定了低温黑暗下发芽的自然变异,并比较了两种生态型Bay-0和Shahdara低温黑暗下的发芽特性,这种自然变异被用来鉴定低温黑暗中发芽基因的遗传位点;利用这两个生态型杂交产生的重组近交系,结合分子标记和QTL方法,检测到种子在低温黑暗下发芽至少受6个QTL所控制,其中3个为主效位点,它们所解释的自然变异为61%,利用异源近亲代(HIF)验证了这3个主要QTL,为低温黑暗发芽基因的精细定位和基因克隆等研究奠定了基础。Low temperature germination is an important agricultural trait.Improvement of crop tolerance to low temperature and identification of target genes are a major goal for crop breeders worldwide.By testing seed germination of over 300 Arabidopsis accessions,we identified a natural variation in germinability at low temperature in the dark among these accessions and compared the germination characteristics between Bay-0 and Shahdara.This natural variation was exploited to identify genetic loci responsible for cold-tolerant and dark germination.The molecular markers and a quantitative trait loci approach were used on recombinant inbred lines of a cross between Bay-0 and Shahdara.Six distinct quantitative trait loci were identified,three of which were major loci responsible for 61%of this natural variation.Validation of the three major loci using heterogeneous inbred families confirmed the feasibility of fine mapping and cloning the genes at the quantitative trait loci responsible for cold-tolerant and dark germination.

关 键 词：拟南芥 低温黑暗发芽 重组近交系 异源近亲代 分子标记 QTL定位 

分 类 号：Q943[生物学—植物学]