荧光定量PCR与常规PCR法检测GⅡ型札幌样病毒的比较  

Comparison of FQ-PCR and conventional RT-PCR method for the detection of GⅡSLVs

作　　者：曾爱华[1] 金小宝 朱家勇 

机构地区：[1]广东药学院药科学院,广东广州510006 [2]广东省生物活性药物研究重点实验室,广东广州510006

出　　处：《中国食品卫生杂志》2012年第3期215-217,共3页Chinese Journal of Food Hygiene

基　　金：广东省科技计划资助项目(2006B36008001)

摘　　要：目的比较荧光定量PCR与常规RT-PCR法检测牡蛎中GⅡ型札幌样病毒的灵敏度与准确性,为该病毒检测提供适用的方法。方法采用荧光定量PCR和RT-PCR方法,分别检测牡蛎样品中的GⅡ型札幌样病毒。结果荧光定量PCR检测的灵敏度可达102拷贝/μl,札幌样病毒呈阳性的牡蛎有6例,阳性率为4.72%(6/127);RT-PCR的灵敏度为103拷贝/μl,札幌样病毒呈阳性的牡蛎有1例,阳性率为0.79%(1/127)。结论与RT-PCR方法比较,荧光定量PCR是更为敏感准确的方法,更适用于牡蛎中GⅡ型札幌样病毒的检测。Objective To compare the sensitivity and accuracy of FQ-PCR and conventional RT-PCR method for the detection of G Ⅱ SLVs in oyster. Methods G Ⅱ SLVs in oyster was detected by FQ-PCR and conventional RT-PCR. Results The sensitivity for the detection of G Ⅱ SLVs in oyster samples by FQ-PCR was 102 copy/μ1 and that by conventional RT-PCR was 103 copy/μ1. The positive rate for FQ- PCR was 4.72% （6/127） and that for conventional RT- PCR was 0. 79% （1/127） （P 〈 0. 05）. Conclusion FQ- PCR is more suitable for the detection of G Ⅱ SLV in oyster.

关 键 词：牡蛎 GⅡ型札幌样病毒 荧光定量PCR 常规RT-PCR 

分 类 号：R450[医药卫生—治疗学]