游动放线菌PDF-1代谢产物中抗耐药菌株活性成分研究  

Purification and characterization of anti multi-drug-resistant bacterial constituents from fermentation broth of Actinoplanes sp.PDF-1

作　　者：李子强[1] 王莉宁[1] 胡辛欣[1] 董国霞[1] 魏玉珍[1] 刘红宇[1] 张玉琴[1] 王玉成[1] 游雪甫[1] 余利岩[1] 

机构地区：[1]中国医学科学院,北京协和医学院,医药生物技术研究所,北京100050

出　　处：《中国抗生素杂志》2012年第5期366-371,共6页Chinese Journal of Antibiotics

基　　金：国家自然科学基金项目(No.30870026);国家微生物资源平台;"重大新药创制"科技重大专项(No.2009ZX09301-003);卫生公益性行业科研(201002021)

摘　　要：目的从一株游动放线菌Actinoplanes sp.PDF-1发酵液中分离抗多种耐药细菌的活性化合物。方法通过活性追踪,利用多种色谱技术手段,结合现代波谱学方法分离鉴定化合物结构。结果从Actinoplanes sp.PDF-1的发酵产物中分离纯化得到6个化合物,诺西肽(Nosiheptide,1)、2′-脱氧胸腺嘧啶核苷(2′-Deoxythymidine,2)、2′-脱氧胞嘧啶核苷(2′-Deoxycytidine,3)、胡萝卜苷(Daucosterol,4)、邻氨基苯甲酸(2-Aminobenzoic acid,5)和N^b-乙酰基色胺(N^b-Acetyltryptamine,6)。结论从Actinoplanessp.PDF-1的发酵产物中分离纯化得到6个化合物,其中化合物l为抗耐药细菌的主要活性成分。Objective To investigate anti multidrug-resistant bacterial constituents from the fermentation broth of Actinoplanes sp. PDF-1. Methods Active constituents were isolated and purified by bioassay-guided isolation and various chromatographic techniques. Structures of the isolates were identified by spectroscopic methods including UV, NMR, 2D-NMR and MS spectra. Results Six compounds were obtained from the fermentation products of Actinoplanes sp. PDF-1. Their structures were elucidated as nosiheptide （1）, 2＇-deoxythymidine （2）, 2＇-deoxycytidine （3）, daucosterol （4）, 2-aminobenzoic acid （5） and Nb-acetyltryptamine （6）. Conclusion The main anti-drug-resistant bacterial substance produced by Actinoplanes sp. PDF-1 was identified as nosiheptide.

关 键 词：游动放线菌属 诺西肽 抗耐药细菌 

分 类 号：R379.1[医药卫生—病原生物学]