正交试验法优选三黄丸提取工艺  

Optimization of Extraction Technology for Sanhuang Pill by Orthogonal Test

作　　者：马燕[1] 喇孝瑾[2] 白素芬[2] 吕建东[2] 张晓鹏[2] 韩刚[2] 王虹玉[2] 喇万英[2] 

机构地区：[1]开滦(集团)有限责任公司医院分院,河北唐山063000 [2]河北联合大学,河北唐山063000

出　　处：《中国实验方剂学杂志》2012年第10期31-33,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：科技部国际科技合作项目(2008DFA31050)

摘　　要：目的:优选三黄丸的提取工艺。方法:以盐酸小檗碱含量为指标,高效液相色谱法测定提取液中盐酸小檗碱含量,采用正交试验设计优选三黄丸的提取工艺。结果:三黄丸的最佳提取工艺为6倍量水提取2次,每次1.5 h。结论:该提取工艺稳定,安全,简便易行。Objective： To optimize extraction technology of Sanhuang pill. Method： 0rthogonal experiment was used to optimize extraction technology of Sanhuang pill with the content of berberine hydrochloride as index, the content of berberine hydrochloride was determined by HPLC. Result： Optimum extraction process of Sanhuang pill was： extracted 2 times with 6 times the amount of water, 1.5 h each time. Conclusion： This optimized technology of Sanhuang pill was simple, stable and effective.

关 键 词：三黄丸 正交试验 提取工艺 高效液相色谱法 盐酸小檗碱 

分 类 号：R283.6[医药卫生—中药学]