RP-HPLC测定不同基源郁金药材中牻牛儿酮的含量  

RP-HPLC Determination of Germacron in Curcumae Radix

作　　者：刘玉红[1] 刘倩伶[1] 李晓亮[1,2] 陈燕[1] 李青苗[1] 易进海[1] 

机构地区：[1]四川省中医药科学院,成都610041 [2]泸州医学院,四川泸州646000

出　　处：《中国实验方剂学杂志》2012年第10期133-136,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药行业专项中药类项目(200807020)

摘　　要：目的:测定4种基源40批郁金药材中牻牛儿酮的含量。方法:采用反相高效液相色谱法,Eclipse XDB-C18(4.6mm×150 mm,5μm)色谱柱,以乙腈-水(60∶40)为流动相,流速1.0 mL·min-1,检测波长210 nm。结果:牻牛儿酮进样量在0.045 8～0.687μg有良好线性关系(r=0.999 9),平均回收率(n=6)为99.2%,牻牛儿酮含量在0.001 26%～0.224%。结论:提供了40批不同基源郁金药材中牻牛儿酮的含量,为科学制定郁金药材的质量标准提供数据支持。Objective： To determinate the content of germacron of 40 batches Curcumae Radix with germacron as control. Method： The amounts of germacron were determinated by RP-HPLC method with Eclipse XDB-C18 （4.6 mm × 150 mm, 5 μm） column as analytical column, 210 nm as detective wavelength, aeetonitrile- water （60：40） as mobile phase at a flow rate of 1.0 mL·min-1. Result： The standard curve of germaeron was linear in the range of 0. 045 8-0. 687 μg （r =0. 999 9） , and the average recovery （n =6） was 99.2% , the range of germacron was 0. 001 26% -0. 224%. Conclusion： We provide the content of germacron of 40 batches of four species of Curcumae Radix is determined to provide data support for establishing scientific quality control standards.

关 键 词：郁金 牻牛儿酮 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]