泽泻麸制前后健脾作用研究  

The Invigorating Spleen Function by Alisma orientalis before and after Processing

作　　者：张宏达[1] 谢雪[1] 陈昱竹[1] 任国杰[1] 王晓玲[1] 许枬[1] 

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《中国实验方剂学杂志》2012年第10期187-190,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家公益性中医药行业科研专项课题(200807039)

摘　　要：目的:对比泽泻炮制前后对大鼠胃泌素含量以及十二指肠Na+-K+-ATP酶活性的影响,探讨泽泻炮制前后健脾作用的变化。方法:取50只雄性SD大鼠随机分为空白组、泽泻生品低剂量组、泽泻生品高剂量组、泽泻麸制品低剂量组、泽泻麸制品高剂量组,空白组ig给予生理盐水,各低、高剂量组每次ig给予对应药物2.0,6.0 g·kg-1。每天ig给药2次,7 d后测定大鼠血清胃泌素水平及十二指肠中Na+-K+-ATP酶活性。采用离体器官实验法,以大鼠离体十二指肠平滑肌的收缩幅度、相对张力及收缩频率为指标,观察在克氏液中泽泻炮制前后提取液对大鼠离体十二指肠平滑肌的影响。结果:泽泻麸制品高剂量组血清胃泌素含量为(3.77±0.49)ng·L-1,明显高于相同剂量生品组(3.24±0.13)ng·L-1(P<0.05);麸制品高剂量组Na+-K+-ATP酶含量为(13.31±1.99)pmol·g-1,明显高于相同剂量生品组(11.77±1.37)pmol·g-1(P<0.05);生品和麸制品在高浓度时,对大鼠离体十二指肠收缩振幅及相对张力都有增强(P<0.05)。结论:泽泻能增加大鼠血清胃泌素含量、提高十二指肠Na+-K+-ATP酶活性,以及大鼠离体十二指肠肠管的运动功能,且呈剂量依赖关系,炮制后作用增强。Objective： To compare the influence of Alisma orientalis gastrin content and Na ＋-K ＋-ATP enzyme activity, and to explore the invigorating spleen function of A. orientalis before and after processing. Method： Fifty healthy male SD rats were divided randomly into the control group, the bown product low dose group, born product high dose group, the processed products low dose group, the processed products high dose group, and control group was given physiological saline, the low dose groups were given corresponding drugs at dose of 2.0 g· kg- 1 , the high dose groups were given corresponding drugs at dose of 6.0 g· kg-1 twice a day for 7 days. Then, the gastrin levels and duodenal Na ＋ -K ＋ -ATP enzyme activity, contraction amplitude, tension and the frequency of the isolated duodenal＇s smooth muscle of rats were measured in the Krebs solutions. Result： The gastrin content of processed products of high dose group was （3.77 ± 0.49） ng· L- 1 , that was higher than that in born product high dose group s （3.24 ±0.13） ng·L-1 （P 〈0.05）. The Na＋-K＋-ATP enzyme activity in processed products of high dose group was （ 13.31± 1.99） pmol· g-1, that was higher than that in born product high dose group （11.77 ± 1.37） pmol· g-1 （P 〈 0.05）. High concentration in processed and born product groups could increase amplitude and relative tension of duodenum （P 〈 0.05）. Conclusion： The influence of A. orientalis on gastrin level, duodena Na＋-K ＋-ATP enzyme and duodenal＇s smooth muscle is obvious, and after processed, and the actions can be enhanced.

关 键 词：泽泻 炮制 健脾作用 胃泌素 NA+-K+-ATP酶 

分 类 号：R285.5[医药卫生—中药学]