茯苓多糖退黄疸作用的实验研究  

Effects of Pachymaran on Alpha-naphthylisothi-induced Jaundice in Rats

作　　者：刘成[1,2] 杨宗国[1] 陆云飞[1,3] 吕震[1] 徐庆年[1] 潘奇[1] 汤伯宗[1] 吕莹[1] 孙媛[1] 陆玲庆[1] 陈晓蓉[1,3] 

机构地区：[1]上海市(复旦大学附属)公共卫生临床中心,中医科,上海201508 [2]上海市(复旦大学附属)公共卫生临床中心,科研中心,上海201508 [3]国家中医药管理局中医药防治传染病重点研究室(临床基地),上海201508

出　　处：《中国实验方剂学杂志》2012年第10期195-198,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十一五"科技重大专项子课题(2009ZX10005-019);上海市科学技术委员会项目(25382);上海市科委引导类项目(10411963000);中央高校基本科研业务费专项资金(10F115)

摘　　要：目的:以异硫氰酸-α-萘酯(alpha-naphthylisothi,ANIT)大鼠黄疸模型为载体,探索茯苓多糖退黄作用。方法:Wistar雄性大鼠50只随机分为正常组(10只)和ANIT处理组(40只),2%的ANIT按照6 mL·kg-1ig,48 h后ANIT处理组大鼠随机分为模型对照组及茯苓多糖低、中、高剂量(5,50,500 mg·kg-1),每组10只。模型大鼠每天ig给予生理盐水。茯苓多糖组ig给药1周,实验结束处死全部大鼠,检测大鼠肝功能及实时定量(real-time PCR)方法检测白介素-1β(IL-1β)及肿瘤坏死因子(TNF-α)水平。结果:ANIT处理组大鼠肝功能紊乱,与正常组相比,血清总胆红素(TBil)含量显著升高(P<0.01),为正常组的52.7倍,谷丙转氨酶(ALT)活性显著升高(P<0.01),为正常组的6.31倍,天冬氨酸转氨酶(AST)活性显著升高(P<0.01),为正常组的8.31倍。茯苓多糖退黄疸保肝作用呈剂量依赖型增高,中剂量组退黄有一定作用,高剂量组退黄作用最为显著。ANIT造模组IL-1β,TNF-αmRNA促炎症因子显著升高(P<0.01),分别为正常组的14.24,10.65倍;而模型组IL-4 mRNA表达仅为正常组的0.33(P<0.01);与模型组相比,高剂量茯苓多糖显著抑制IL-1β,TNF-α的mRNA表达(P<0.01),茯苓多糖高剂量组显著提高IL-4 mRNA表达(P<0.01)。结论:高剂量茯苓多糖可能通过免疫调节发挥退黄作用。Objective： To investigate the effects of pachymaran on elimination of jaundice. Method：Fifty male Wistar rats were randomly divided into normal group （10） and alpha-naphthylisothi （ANIT） -induced （40） model group. After 48 h of ANIT treatment, ANIT-treated rats were randomly divided into model group,pachymaran low-dose, medium-dose and high-dose groups （10 rats each group）. At the end of the experiment all rats were sacrificed. Liver function, pro-inflammatory and anti-inflammatory cytokines in liver were determined using real-time PCR analysis. Result： Compared with normal group： the acitivity of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in the serum increased 6.31-fold and 8.31-fold, respectively （P 〈 0.05 or P 〈0.0. 1 ）. The content of total bilirubin （TBil） increased 52.7-fold （P 〈0.05 or P 〈0. O. 1 ） in ANIT- treated rats. Compared with the model group, pachymaran high-dose reduced significantly TBil content （P 〈 0.01）. Compared with normal group, linterleukin-1 beta （IL-1/3） and tumor necrosis factor （TNF-a） mRNA levels increased significantly （P 〈 0.01 ） and IL-4 was decreased remarkably （P 〈 0.05） in ANIT-treated rats, while the pachymaran high-dose reduced IL-1/3 and TNF-a mRNA expression （P 〈 0. 05 or P 〈 0. 1 ） , elevated liver IL-4 expression （P 〈 0. 05）. Conclusion： Could be one important mechanism for pachymaran can reduce jaundice by regulating immunologic function in rats.

关 键 词：茯苓多糖 异硫氰酸-α-萘酯 黄疸 

分 类 号：R285.5[医药卫生—中药学]