暗紫贝母、栽培瓦布贝母及浙贝母药效学比较  

Pharmacodynamic Compariason of Fritillaria unibracteata,Fritillaria unibracteata var.wabensis and Fritillaria thunbergii Cultivated

作　　者：颜晓燕[1,2] 童志远[3] 晏子俊[2] 罗燕秋[2] 唐丽[2] 吴珊珊[2] 杨军[2] 彭成[1] 

机构地区：[1]成都中医药大学药学院,成都610075 [2]成都医学院药学院,成都610083 [3]成都医学院第一附属医院,四川成都610513

出　　处：《中国实验方剂学杂志》2012年第10期244-248,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：灾区药材资源恢复重建与综合开发利用研究及示范"十一五"国家科技支撑计划重点项目(2009BAI84B00)

摘　　要：目的:比较暗紫贝母、栽培瓦布贝母及浙贝母镇咳、祛痰及平喘作用,明确栽培瓦布贝母作为野生川贝母替代资源的可行性。方法:采用小鼠氨水引咳及豚鼠枸橼酸引咳法比较3种贝母的镇咳作用。小鼠酚红排痰法及大鼠毛细管排痰实验法比较3种贝母的祛痰作用;整体动物引喘实验法比较3种贝母的平喘作用。每个实验均分为5个组,分别为阴性对照组,阳性对照组,暗紫贝母组,栽培瓦布贝母组及浙贝母组。阴性组采用蒸馏水或生理盐水灌胃5 d。镇咳实验中3种贝母的剂量分别为2.5 g·kg-1·d-1(小鼠),1.5 g·kg-1·d-1(豚鼠);祛痰实验中3种贝母的剂量分别为2 g·kg-1·d-1(小鼠),1 g·kg-1·d-1(大鼠),平喘实验中3种贝母的剂量为1.5 g·kg-1·d-1。结果:栽培瓦布贝母祛痰作用与暗紫贝母相当,小鼠酚红排泌量与阴性对照组比较,P<0.01,大鼠毛细管排泌量与阴性对照组比较P<0.05,栽培瓦布贝母平喘作用与暗紫贝母相当,哮喘潜伏期与阴性对照组比较,P<0.05,暗紫贝母与栽培瓦布贝母作用差异无统计学意义;栽培瓦布贝母镇咳作用与暗紫贝母及浙贝母相当,减少枸橼酸引咳后5 min内咳嗽次数与阴性组比较,差异有统计学意义。结论:栽培瓦布贝母可作为野生川贝母的替代资源推广种植与应用。Objective： Experiments were carried on to compare the antitussive, expectorant and antiasthmatic effects of Fritillaria unibracteata, F. unibracteata var. wabensis and F. thunbergii and identify if the F. unibracteata, wabensis can be used as the alternative resources of wild F. cirrhosa. Method： The antitussive effects of the 3 kinds of fritillarias s were investigated through testing the cough times and EDT50 induced by ammonium hydroxide in mice and the counts of cough induced by critic acid in guinea pigs. The expectorant activities were sdudied by testing the amount of phlegm secreted in mice and rats. The antiasthma effect was studied by the asthma time induced by acetylcholine chloride and histamine phosphate in guinea pigs. There were negative control, positive control and 3 kinds of fritillarias groups in every experiment. The dose of fritillarias in the antitussive experiment was 2.5 g· kg^-1 ·d^-1 in mice and 1.5 g· kg^-1 ·d^-1 in guinea pigs. The dose of 3 fritillarias in the expectorant experiment was 2 g· kg^-1 ·d^-1 in mice and 1 g· kg^-1 ·d^-1 in rats, The dose of 3 kinds of fritillarias in the anti-asthma experiment was 1.5 g· kg^-1 ·d^-1. The administration time of every experiments was 5 days. Result： F. unibracteata var. wabensis cultivated has similar eliminating phlegm activities and antiasthma effects to F. unibracteata. It also has similar antitussive effects to F. unib-racteata and F. thunbergii Miq. Conclusion： F. unibract-eata var. wabensis cultivated can be used as alternative resources of wild F. cirrhosa .

关 键 词：暗紫贝母 栽培互布贝母 浙贝母 栽培品 镇咳 祛痰 平喘 

分 类 号：R285.5[医药卫生—中药学]