山茱萸多糖诱导宫颈癌细胞凋亡及Bax蛋白表达的变化  

The Expression of Bax Proteinum of Fructus Corni Polysaccharides in Human Cervical Cancer Cells

作　　者：王恩军[1] 靳祎[1] 王哲[2] 杨欢欢[1] 周艳芬[1] 刘斌[3] 

机构地区：[1]河北大学,河北保定071000 [2]保定市第二医院,河北保定071051 [3]保定市急救中心医院,河北保定071000

出　　处：《中国实验方剂学杂志》2012年第10期260-262,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省人口和计划生育委员会科研计划项目(2009-B18);保定市科技局科学技术研究与发展指导计划项目(09ZF011)

摘　　要：目的:探讨山茱萸多糖诱导人宫颈癌Hela细胞的凋亡作用及Bax蛋白表达的变化。方法:用12.5,25,50 g·L-1的山茱萸多糖作用Hela细胞24 h,倒置显微镜下观察细胞凋亡的形态,免疫组化SP法检测凋亡相关蛋白Bax表达的变化。结果:Hela细胞经3个不同浓度的山茱萸多糖作用后,出现典型的细胞凋亡的形态学变化,其程度与浓度相关;而且Bax蛋白的表达量随浓度升高而增加,免疫组化评分(IHS)分别为0.18,0.44,1.35。结论:山茱萸多糖能够促进Bax的表达,诱导Hela细胞凋亡。Objective： To expLore the apoptosis effect of fructus corni polysaccharides on expression of Bax in Hela cells. Method： Hela cells were treated with various concentration of fructus corni polysaceharides for 24 h, the change of cells＇ apoptosis shape was observed by upside-down microscope, and expression of Bax was detected by immunohistochemistry streptaridin peroxidase. Result： Morphological observation appeared representative cell apoptosis and Bax expression up after fruetus corni polysaccharides on Hela cells. Its degree was correlated with concentration. Conclusion： Fructus eorni polysaccharides can promote Bax expression and induce cell apoptosis.

关 键 词：山茱萸多糖 宫颈癌 HELA细胞 BAX 凋亡 免疫组化 

分 类 号：R285.5[医药卫生—中药学]