TiO_2-石墨烯-Nafion复合膜修饰玻碳电极同时测定多巴胺和尿酸  

Simultaneous Determination of Dopamine and Uric Acid Based on TiO_2-Graphene-Nafion Composite Film Modified Glassy Carbon Electrode

作　　者：刘雪[1] 王兰[1] 樊阳[1] 刘凤杰[1] 

机构地区：[1]信阳师范学院化学化工学院,信阳464000

出　　处：《化学通报》2012年第5期458-462,共5页Chemistry

基　　金：河南省高校科技创新人才基金项目(2010HASTIT025);河南省科技计划项目(112300410217)资助

摘　　要：利用在玻碳电极上修饰了TiO2-石墨烯-Nafion复合膜制得的修饰电极进行多巴胺(DA)和尿酸(UA)的同时测定。用循环伏安法(CV)和差分脉冲伏安法(DPV)研究了该修饰电极的电化学行为。在pH为7.0的磷酸盐缓冲液(PBS)中,修饰电极对于DA和UA的电化学氧化具有良好的电催化性能。DA和UA的氧化峰电流分别在2～120和60～300μmol/L浓度范围内呈良好的线性关系,检出限分别为0.066和0.102μmol/L。实验结果表明,TiO2-石墨烯-Nafion复合膜修饰电极显著提高了检测的灵敏度,并表现出良好的选择性和重现性。A modified electrode, which was fabricated by casting TiO2-graphene-Nafion composite film on glass carbon electrode surface and drying in room temperature, was used for determination of dopamine （DA） and uric acid （UA） simultaneously. The electrochemical behavior of DA and UA at the modified electrode was investigated by cyclic voltametry （CV） and different pulse vohametry （ DPV）. In pH 7.0 phosphate buffer solution （PBS）, the modified electrode showed excellent electro-catalytic activity for the oxidation of DA and UA. The oxidation peak current showed good linear relationships with the concentration of DA and UA in the range of 2 - 120 and 60 - 300p^mol/L, respectively. The limits of detection were 0. 066 and 0. 102μmol/L. The presented method exhibits high sensitivity, selectivity and reproducibility.

关 键 词：TiO2-石墨烯-Nafion复合膜 差分脉冲伏安法 多巴胺 尿酸 

分 类 号：R91[医药卫生—药学] O657.1[理学—分析化学]